The ICCB-Longwood Mechanism of Action Library (ICCB-L MoA) is a growing collection of well-annotated, high quality compounds with known biological targets. A tool score, described by Wang and colleagues (Cell Chemical Biology 23, 862-874), was utilized to identify the best target-compound pairs based upon target specificity and potency, with Rank 1 being the highest. This collection, when complete, will also include Rank 2 and Rank 3 compounds for some biological targets.

The curation of this library was made possible through the support of HMS LINCS and guidance from Yuan Wang and Jeremy Jenkins at Novartis Institutes for BioMedical Research. Compounds were purchased by ICCB-Longwood and the Assay Development & Screening Facility at Boston Children's Hospital, and funded in part by a grant to BCH from Massachusetts Life Sciences Center. Harvard Medical School's Foundry Award Program also provided funds for the acquisition of this library.

Note that 8 wells contain fluorescein (3724:C17, 3724:C18, 3724:D17, 3724:D18, 3732:I07, 3732:I08, 3732:J07, and 3732:J08 ), a highly fluorescent dye with a bright yellowish-green emission that may produce false results in fluorescence and absorbance assays.

Each compound is plated at 4 concentrations (10 mM, ~2 mM, ~0.4 mM and ~0.08 mM). The concentrations of the compounds on this library plate are according to quadrant; Quadrant A1 is at 10 mM, Quadrant A2 is at ~2 mM, Quadrant B1 is at ~0.4 mM and Quadrant B2 is at ~0.08 mM, please see map below for a visual layout.

Plates 3720-3724 were plated in October 2017 and contain 400 Rank 1 compounds. Plates 3725-3728 were plated in February 2018 and contain 320 Rank 1 and 2 compounds. Plates 3729 and 3730 were plated in September 2018 and contain Rank 1, 2 and 3 compounds. Plates 3731-3735 were plated in October 2020 and contain Rank 1, 2 and 3 compounds.

384-well Plate Format