Pin Transfer
ICCB-Longwood utilizes two different robotic platforms to conduct pin transfer of nanoliter volumes from compound storage plates into assay plates using stainless steel pin arrays (made by V&P Scientific).
- 96- and 384-well pin arrays
- Transfer compounds into 96-, 384- and 1536-well microplates
- 384-well assay plate volume 30 uL - calibrated to transfer 33 nL, 100 nL or 300 nL
- 384-well assay plate volume 10 uL - calibrated to transfer 33 nL or 100 nL
- The standard transfer volume for chemical screens in 384-well is 100 nL.
96-well assay plate volume 150 uL - calibrated to transfer 200 nL, 250 nL or 300 nL
Pin Arrays & Calibration provides more information on the pin arrays, the calibration process and selecting the appropriate pin array for your screen.
K2 Compound Transfer Robot (SGM 611)
- Custom pin transfer station featuring an Epson T6-602s SCARA robot designed and built at ICCB-Longwood
- Paired with a Peak Robotics KX2 robot arm to deliver library and assay plates to and from the deck for transfer
- Automated rotating CS6 carousel for microplate hotel racks
- Custom Arduino-based relay board with 24V power supply to switch I/O devices
- Automatic pin tool attachment/detachment
K2 Specifications
Pin Array Calibration
Pin Array Wash
Sonic Compound Transfer Robot (SGM 612)
- Custom pin transfer station featuring an Epson T6-B602s SCARA robot designed and built at ICCB-Longwood
- Paired with a Peak Robotics KX2-Plus robot arm to deliver library and assay plates to and from the deck for transfer
- Automated rotating CS6 carousel for microplate sequential racks
- Custom Arduino-based relay board with 24V power supply to switch I/O devices
- Automatic pin tool attachment/detachment