Fees

A fee is charged for access to Functional Genomics screening at ICCB-Longwood. This fee helps us to recover a portion of our operating expenses. ICCB-Longwood screening fees (and associated Facilities and Administration fees) are revised annually based on the Harvard Fiscal Year (July 1 – June 30). The fee structure is updated on the ICCB-Longwood website as soon as it is available. All charges are based on the screening fee at the time of service, not a previously provided estimate or price structure from a previous fiscal year.

Fees below are for screening activities carried out in FY20 (July 1, 2019 – June 30, 2020).

Note that fees charged for access to ICCB-Longwood do not include the cost of assay reagents and supplies. Funding for all assay reagents and supplies is provided by the screener.

Fees for HMS Internal Quad Screens

Screening Milestone
Fees
Functional Genomics screen set-up fee
$4,534
Additional fee for screening full Synthego Druggable sgRNA CRISPR library
$6,670
Additional fee for screening one full genome siRNA library
$15,179
Additional fee for screening lncRNA siRNA library

$1,610

Additional fee for screening each miRNA reagent library

$1,150

In lieu of screening the full druggable sgRNA CRISPR library, it is possible to screen smaller, focused libraries:  
 Additional fee for screening: Kinases $920
In lieu of screening the full human genome siRNA library, it is possible to screen smaller, focused libraries:  
 Additional fee per library for screening: Phosphatases, Ubiquitin Conjugating Enzymes #1 or Ubiquitin Conjugating Enzymes #2 $230
 Additional fee per library for screening: G-protein Coupled Receptors, Ion Channels, Proteases or Ubiquitin Conjugating Enzymes #3  $460
 Additional fee for screening: Kinases $690

The above full-genome siRNA screening fee includes cherry picks for 1,200 individual siRNA duplexes (representing 300 genes) for each screen. Additional cherry picks can be requested at $2 per duplex picked ($8 per gene). Cherry picks can only be requested for individual duplexes, not for SMARTpool reagents. It is not possible to request a cherry pick from Human Pilot 1.

The above full druggable sgRNA CRISPR fee includes a cherry pick of 150 sgRNAs for each screen.

Fees for HMS-Affiliated and Non-Harvard Academic Screens

Screening Milestone
Fees 
Corresponding F&A
RNAi screen set-up fee
$4,534
$1,083
Additional fee for screening full Synthego Druggable sgRNA CRISPR library
$6,670
$1,595
Additional fee for screening one full genome siRNA library
$15,179
$3,627
Additional fee for screening lncRNA siRNA library

$1,610

$385

Additional fee for screening each miRNA reagent library

$1,150

$275

IIn lieu of screening the full druggable sgRNA CRISPR library, it is possible to screen smaller, focused libraries:
 Additional fee per library for screening: Kinases $920  $220
In lieu of screening the full human genome library, it is possible to screen smaller, focused libraries:
 Additional fee per library for screening: Phosphatases, Ubiquitin Conjugating Enzymes #1 or Ubiquitin Conjugating Enzymes #2 $230  $55
 Additional fee per library for screening: G-protein Coupled Receptors, Ion Channels, Proteases or Ubiquitin Conjugating Enzymes #3 $460  $110
 Additional fee for screening: Kinases  $690  $165

The above full-genome siRNA screening fee includes cherry picks for 1,200 individual siRNA duplexes (representing 300 genes) for each screen. Additional cherry picks can be requested at $2 per duplex picked ($8 per gene). Cherry picks can only be requested for individual duplexes, not for SMARTpool reagents. It is not possible to request a cherry pick from Human Pilot 1.

The above full druggable sgRNA CRISPR fee includes a cherry pick of 150 sgRNAs for each screen.

Additional Information on Functional Genomics Fees

Fees will begin to be assessed once work is initiated at ICCB-Longwood. For screens originating from non-HMS Quad investigator labs (this includes HMS Affiliates whose labs are not located on the HMS Quadrangle and Non-Harvard investigators), the indicated F&A is added at each stage of the screening fee. The fee for screening Functional Genomics libraries will be assessed when the first library plate is screened.

The above fees for screening the full siRNA genome include cherry picks for the individual siRNA duplexes corresponding to 300 genes per screen (1200 duplexes for Dharmacon genome, 900 duplexes for an Ambion screen). Additional cherry picks can be requested at $2 per duplex picked ($8 per gene). Cherry picking will only be carried out for individual duplexes, not for SMARTpool reagents. Cherry picking can only be requested after primary screening of SMARTpool libraries. The duplex volume provided is the same volume of SMARTpool reagents that were used in the primary screen.

The above full druggable sgRNA CRISPR fee includes a cherry pick of 150 sgRNAs for each screen.

It is possible for investigators to select whether they would like to screen the entire genome or instead screen focused siRNA libraries. For the human genome, this decision should be made prior to initiating the screen, as the most concise way to screen the entire genome is through utilizing the continuous human genome siRNA library. Custom siRNA library screening is also possible.

Interested investigators should contact Jennifer Smith for more details and/or to determine which rate applies to their screen.