Fees

A fee is charged for access to RNAi screening at ICCB-Longwood. This fee helps us to recover a portion of our operating expenses. ICCB-Longwood screening fees (and associated Facilities and Administration fees) are revised annually based on the Harvard Fiscal Year (July 1 – June 30). The fee structure is updated on the ICCB-Longwood website as soon as it is available. All charges are based on the screening fee at the time of service, not a previously provided estimate or price structure from a previous fiscal year.

Fees below are the anticipated rates for screening activities carried out in FY19 (July 1, 2018 – June 30, 2019); they are awaiting School approval to be finalized.

Note that fees charged for access to ICCB-Longwood do not include the cost of screening reagents and supplies. Funding for all screening reagents and supplies is provided by the screener.

Fees for HMS Internal Quad Screens

Screening Milestone
Fees
RNAi screen set-up fee
$4,385
Additional fee for screening one full genome siRNA library
$14,681
Additional fee for screening lncRNA siRNA library

$1,554

Additional fee for screening each miRNA reagent library

$1,110

In lieu of screening the full human genome library, it is possible to screen smaller, focused libraries:  
 Additional fee per library for screening: Phosphatases, Ubiquitin Conjugating Enzymes #1 or Ubiquitin Conjugating Enzymes #2 $222
 Additional fee per library for screening: G-protein Coupled Receptors, Ion Channels, Proteases or Ubiquitin Conjugating Enzymes #3  $444
 Additional fee for screening: Kinases $666

The above full-genome screening fee includes cherry picks for 1,200 individual siRNA duplexes (representing 300 genes) for each screen. Additional cherry picks can be requested at $2 per duplex picked ($8 per gene). Cherry picks can only be requested for individual duplexes, not for SMARTpool reagents. It is not possible to request a cherry pick from Human Pilot 1.

Fees for HMS-Affiliated and Non-Harvard Academic Screens

Screening Milestone
Fees 
Corresponding F&A
RNAi screen set-up fee
$4,385
$1,054
Additional fee for screening one full genome siRNA library
$14,681
$3,528
Additional fee for screening lncRNA siRNA library

$1,554

$374

Additional fee for screening each miRNA reagent library

$1,110

$267

In lieu of screening the full human genome library, it is possible to screen smaller, focused libraries:
 Additional fee per library for screening: Phosphatases, Ubiquitin Conjugating Enzymes #1 or Ubiquitin Conjugating Enzymes #2 $222  $53
 Additional fee per library for screening: G-protein Coupled Receptors, Ion Channels, Proteases or Ubiquitin Conjugating Enzymes #3 $444  $106
 Additional fee for screening: Kinases  $666  $159

The above full-genome screening fee includes cherry picks for 1,200 individual siRNA duplexes (representing 300 genes) for each screen. Additional cherry picks can be requested at $2 per duplex picked ($8 per gene). Cherry picks can only be requested for individual duplexes, not for SMARTpool reagents. It is not possible to request a cherry pick from Human Pilot 1.

Additional Information on RNAi Fees

Fees will begin to be assessed once work is initiated at ICCB-Longwood. For screens originating from non-HMS Quad investigator labs (this includes HMS Affiliates whose labs are not located on the HMS Quadrangle and Non-Harvard investigators), the indicated F&A is added at each stage of the screening fee. The fee for screening RNAi libraries will be assessed when the first library plate is screened.

The above fees for screening the full genome include cherry picks for the individual siRNA duplexes corresponding to 300 genes per screen (1200 duplexes for Dharmacon genome, 900 duplexes for an Ambion screen). Additional cherry picks can be requested at $2 per duplex picked ($8 per gene). Cherry picking will only be carried out for individual duplexes, not for SMARTpool reagents. Cherry picking can only be requested after primary screening of SMARTpool libraries. The duplex volume provided is the same volume of SMARTpool reagents that were used in the primary screen.

It is now possible for investigators to select whether they would like to screen the entire genome or instead screen focused libraries. For the human genome, this decision should be made prior to initiating the screen, as the most concise way to screen the entire genome is through utilizing the continuous human genome library. Custom library screening is also supported as possible.

Interested investigators should contact Jennifer Smith for more details and/or to determine which rate applies to their screen.