Fees will begin to be assessed once work is initiated at ICCB-Longwood. For screens originating from non-HMS Quad investigator labs (this includes HMS Affiliates whose labs are not located on the HMS Quadrangle and Non-Harvard investigators), the indicated F&A is added at each stage of the screening fee. The fee for screening Functional Genomics libraries will be assessed when the first library plate is screened.
The above fees for screening the full siRNA genome include cherry picks for the individual siRNA duplexes corresponding to 300 genes per screen (1200 duplexes for Dharmacon genome, 900 duplexes for an Ambion screen). Additional cherry picks can be requested at $4 per duplex picked ($16 per gene). Cherry picking will only be carried out for individual duplexes, not for SMARTpool reagents. Cherry picking can only be requested after primary screening of SMARTpool libraries. The duplex volume provided is the same volume of SMARTpool reagents that were used in the primary screen.
The above full druggable sgRNA CRISPR fee includes a cherry pick of 150 sgRNAs for each screen.
It is possible for investigators to select whether they would like to screen the entire genome or instead screen focused siRNA libraries. For the human genome, this decision should be made prior to initiating the screen, as the most concise way to screen the entire genome is through utilizing the continuous human genome siRNA library. Custom siRNA library screening is also possible.
Interested investigators should contact Jennifer Smith for more details and/or to determine which rate applies to their screen.