The stock concentration for arrayed synthetic single-guide RNAs (sgRNA) CRISPR knock out library plates designated for screening is 1 µM. All of the sgRNAs are stored in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0).
Each well contains up to three sgRNA targetting the same gene (>93% have three guides/target)
Synthego Human Arrayed sgRNA CRISPR-Cas9
| Library Name | Number of siRNA Wells | Number of Plates | ICCB-L Plate Numbers |
|---|---|---|---|
| Synthego sgRNA Human Druggable Genome | 8,478 | 29 | 51,490 - 51,518 |
| Synthego sgRNA Human Kinase | 1,035 | 4 | 51,519 - 51,522 |