Synthego sgRNA Human Druggable Genome

This collection of Synthego synthetic single-guide RNAs (sgRNA) was purchased in 2019 and plated in January 2020. The design of this library is based on Gencode Release 26 (GRCh38.p10). It incorporates recent advances in sgRNA algorithm design to decrease off-target effects and increase the probability of an indel resulting in gene knock out.