Relevant Supplies

Many of the items listed in the tables below are available to purchase from the screening facility on an as needed basis. If you are an active ICCB-L screening user, please contact the Screening Facility Staff to request information on current pricing and availability of a particular item. 

Assay Plates

 

384-well Plate Type Corning Cat. #  Assay Volume (uL)Bottom TypeRecommended Assay Type(s)
Clear, TC 3701 20-80 Clear, Polystyrene Absorbance
Black, TC 3764 20-80 Clear, Polystyrene Imaging
Black, S 3664 20-80 Clear, Poly-D-Lysine Imaging
Black, S 3683 20-80 Clear, CellBind Imaging
White, TC 3570 20-80 Opaque Luminescence
Black, TC 3571 20-80 Opaque Luminescence

Intermediate Assay Plates

384-well Plate Type

Eppendorf Cat. #

Assay Volume (uL)

Bottom Type

twin.tec clear walls 12000-658 2-30 V bottom
twin.tec yellow walls 12000-660 2-30 V bottom
twin.tec red walls 12000-666 2-30 V bottom

Manifolds and Aspiration Wands

Instrument Supplier Cat.# Item Description Image
Themo Combi Thermo-Fisher 24072670 8-channel Manifold, Standard-Bore, 5-2,500uL
24073290 8-channel Manifold, Small-Bore, plastic tip 0.5-50uL
24073295 8-channel Manifold, Small-Bore, metall tip, 0.5-50uL
Aspirator Wand V&P Scientific VP 186L Polycarbonate 24-chanel. Manually aspirate volume from a 384-well assay plate
Aspirator Wand Drummond 3-000-01 Stainless steel 24-channel. Manually aspirate volume from a 384-well assay plate

HTS Assay Kits

Item Description

Cat. #

Manufacturer

Assay Type

ATPLite 6016943 PerkinElmer Luminescent Cell Viability
ADP-Glo (100,000 Assay) V9103 Promega
Kinase Assay System
BrightGlo (100mL) E2620 Luciferase Assay System
BrightGlo (10 x 100mL) E2650
DualGlo (100mL) E2940
DualGlo (10 x 100mL) E2980
SteadyGlo (100mL) E2520
SteadyGlo (10 x 100mL) E2550
CellTiterGlo (100mL) G7572 Luminescent Cell Viability
CellTiterGlo (10 x 100mL) G7573

Plate Seals

Item Type

Cat. #

Manufacturer

Item Description

AbsorbMax T896101 E&K Scientific Black sealing film, non-sterile
T896101S Black sealing film, sterile
Breathable Assay Seal 241205 NUNC To seal assay plates during incubation to reduce evaporation
Aluminum DMSO resistant 6570 Corning Used to seal assay plates with fixed cells, before  IXM readout
MicroClime Environmental Lid LL-0310 LabCyte Plate lid with chamber to cover assay plates during incubation to reduce evaporation
Opaque Bottom Covers 6005189 PerkinElmer Black (apply to clear bottom plates to read in plate reader)
6005199 White (apply to clear bottom plates to read in plate reader)
Temporary Assay Seal 19570 Qiagen To seal assay plates during incubation to reduce evaporation

Library Storage

RNAi Library Plates

384-well Plate Type

Cat. #

Manufacturer

Assay Volume (uL)

Bottom Type

sterile, RNAse/DNAse free, polypropylene 3656 Corning 95 round
twin.tec blue walls 12000-664 Eppendorf 2-30 V bottom
twin.tec green walls 12000-662 Eppendorf 2-30 V bottom

Reagents and Tips

Item

Supplier

Cat. #

Item Description

Nuclease-free water Qiagen 129114 10 bottles of 50mL each
Nuclease-free water Qiagen 129115 1000mL bottle
Nuclease-free water Qiagen 129117 Box of 5L
96-tip racks Agilent/ Fluotics 06880.002 200uL, non-sterile
96-tip racks Agilent/ Fluotics 08585.002 180uL, Sterile, Filtered
384-tip racks Agilent/ Fluotics 11484.002 30uL, non-sterile