Many of the items listed in the tables below are available to purchase from the screening facility on an as needed basis. If you are an active ICCB-L screening user, please contact the Screening Facility Staff to request information on current pricing and availability of a particular item.
Relevant Supplies
Assay Plates
384-well Plate Type | Corning Cat. # | Assay Volume (uL) | Bottom Type | Recommended Assay Type(s) |
---|---|---|---|---|
Clear, TC | 3701 | 20-80 | Clear, Polystyrene | Absorbance |
Black, TC | 3764 | 20-80 | Clear, Polystyrene | Imaging |
Black, S | 3664 | 20-80 | Clear, Poly-D-Lysine | Imaging |
Black, S | 3683 | 20-80 | Clear, CellBind | Imaging |
White, TC | 3570 | 20-80 | Opaque | Luminescence |
Black, TC | 3571 | 20-80 | Opaque | Luminescence |
Intermediate Assay Plates
384-well Plate Type |
Eppendorf Cat. # |
Assay Volume (uL) |
Bottom Type |
---|---|---|---|
twin.tec clear walls | 12000-658 | 2-30 | V bottom |
twin.tec yellow walls | 12000-660 | 2-30 | V bottom |
twin.tec red walls | 12000-666 | 2-30 | V bottom |
Manifolds and Aspiration Wands
Instrument | Supplier | Cat.# | Item Description | Image |
Themo Combi | Thermo-Fisher | 24072670 | 8-channel Manifold, Standard-Bore, 5-2,500uL | |
24073290 | 8-channel Manifold, Small-Bore, plastic tip 0.5-50uL | |||
24073295 | 8-channel Manifold, Small-Bore, metall tip, 0.5-50uL | |||
Aspirator Wand | V&P Scientific | VP 186L | Polycarbonate 24-chanel. Manually aspirate volume from a 384-well assay plate | |
Aspirator Wand | Drummond | 3-000-01 | Stainless steel 24-channel. Manually aspirate volume from a 384-well assay plate |
HTS Assay Kits
Item Description |
Cat. # |
Manufacturer |
Assay Type |
---|---|---|---|
ATPLite | 6016943 | PerkinElmer | Luminescent Cell Viability |
ADP-Glo (100,000 Assay) | V9103 | Promega |
Kinase Assay System |
BrightGlo (100mL) | E2620 | Luciferase Assay System |
|
BrightGlo (10 x 100mL) | E2650 | ||
DualGlo (100mL) | E2940 | ||
DualGlo (10 x 100mL) | E2980 | ||
SteadyGlo (100mL) | E2520 | ||
SteadyGlo (10 x 100mL) | E2550 | ||
CellTiterGlo (100mL) | G7572 | Luminescent Cell Viability |
|
CellTiterGlo (10 x 100mL) | G7573 |
Plate Seals
Item Type |
Cat. # |
Manufacturer |
Item Description |
---|---|---|---|
AbsorbMax | BK-50 | Excel Scientific | Black sealing film, non-sterile |
Breathable Assay Seal | 241205 | NUNC | To seal assay plates during incubation to reduce evaporation |
Aluminum DMSO resistant | 6570 | Corning | Used to manually seal assay or compound plates |
MicroClime Environmental Lid | LL-0310 | LabCyte | Plate lid with chamber to cover assay plates during incubation to reduce evaporation |
Opaque Bottom Covers | 6005189 | PerkinElmer | Black (apply to clear bottom plates to read in plate reader) |
6005199 | White (apply to clear bottom plates to read in plate reader) | ||
Temporary Assay Seal | 19570 | Qiagen | To seal assay plates during incubation to reduce evaporation |
BrandTech Brand Self-adhesive | 701371 | BrandTech | Vinyl material; to seal assay plates during storage or taking fluorescence measurements |
Library Storage
RNAi Library Plates
384-well Plate Type |
Cat. # |
Manufacturer |
Assay Volume (uL) |
Bottom Type |
---|---|---|---|---|
sterile, RNAse/DNAse free, polypropylene | 3656 | Corning | 95 | round |
twin.tec blue walls | 12000-664 | Eppendorf | 2-30 | V bottom |
twin.tec green walls | 12000-662 | Eppendorf | 2-30 | V bottom |
Reagents and Tips
Thermo Functional Genomics Quote for ICCB-L Investigators. ICCB-L RNAi screeners interested in the current Dharmacon quote should contact Jen Smith.
Item |
Supplier |
Cat. # |
Item Description |
---|---|---|---|
Nuclease-free water | Qiagen | 129114 | 10 bottles of 50mL each |
Nuclease-free water | Qiagen | 129115 | 1000mL bottle |
Nuclease-free water | Qiagen | 129117 | Box of 5L |
96-tip racks | Agilent/ Fluotics | 06880.002 | 200uL, non-sterile |
96-tip racks | Agilent/ Fluotics | 08585.002 | 180uL, Sterile, Filtered |
384-tip racks | Agilent/ Fluotics | 11484.002 | 30uL, non-sterile |