The stock concentration for the siRNA library plates designated for screening is 1 µM. All of the siRNAs are stored in 1x Dharmacon siRNA buffer (20 mM KCl, 6 mM HEPES-pH 7.5, and 0.2 mM MgCl2). Screeners using 96-well plates for their assay will be provided with the appropriate information to translate 384-well plate numbers to 96-well plate numbers for the purposes of labeling assay plates.
Dharmacon 2014 Human Pools – Continuous Human Genome
Library Name | Number of siRNA Wells | Number of Plates | ICCB-L Plate Numbers |
---|---|---|---|
Human 6 - Druggable Genome Compiled 9-16 Focused Libraries | 2,785 | 10 | 51,144 - 51,153 |
Human 7 - Remaining Druggable | 4,853 | 18 | 51,154 - 51,171 |
Human 8 - Remaining Genome | 10,582 | 38 | 51,172 - 51,209 |
237 | 1 | 51,083 |
Dharmacon 2014 Human siRNA Pools – Focused Libraries
Library Name | Number of siRNA Wells | Number of Plates | ICCB-L Plate Numbers |
---|---|---|---|
Human 9 – G-protein Coupled Receptors | 390 | 2 | 51,210 - 51,211 |
Human 10 - Kinases | 720 | 3 | 51,212 - 51,214 |
Human 11 - Ion Channels | 349 | 2 | 51,215- 51,216 |
480 | 2 | 51,217- 51,218 | |
256 | 1 | 51,219 | |
89 | 1 | 51,220 | |
115 | 1 | 51,221 | |
390 | 2 | 51,222 - 51,223 |
Dharmacon 2014 Human lncRNA siRNA Pools
Library Name | Number of siRNA Wells | Number of Plates | ICCB-L Plate Numbers |
---|---|---|---|
Human 17 - Lincode NR Long-noncoding RNA | 2,231 | 7 | 51,224 - 51,230 |
Ambion Human siRNA Pools
Library Name | Number of siRNA Wells | Number of Plates | ICCB-L Plate Numbers |
---|---|---|---|
Ambion Human 1 - Druggable Genome | 10,414 | 36 | 50,711 - 50,746 |
Ambion Human 2 - Remaining Genome | 11,170 | 38 | 50,747 - 50,784 |
If you have screened a library at ICCB-L that you cannot find on these lists, please check the retired libraries list.