The stock concentration for the siRNA library plates designated for screening is 1 µM. All of the siRNAs are stored in 1x Dharmacon siRNA buffer (20 mM KCl, 6 mM HEPES-pH 7.5, and 0.2 mM MgCl2). Screeners using 96-well plates for their assay will be provided with the appropriate information to translate 384-well plate numbers to 96-well plate numbers for the purposes of labeling assay plates.
| Library Name | Number of siRNA Wells | Number of Plates | ICCB-L Plate Numbers |
|---|---|---|---|
| Mouse 1 - Kinases and Phosphatases | 719 | 3 | 50,025 - 50,027 |
| Mouse 2 - GPCRs | 474 | 2 | 50,028 - 50,029 |
| Mouse 3 - Remaining Druggable Genome | 5,170 | 17 | 50,030 - 50,046 |
| 10,510 | 33 | 50,440 - 50,472 | |
| 3,679 | 14 | 51,084 - 51,097 |
If you have screened a library at ICCB-L that you cannot find on this list, please check the retired libraries list.