EnVision

Revvity EnVision plate reader

ICCB-Longwood has three EnVision plate readers that can be run in single plate mode or with a detachable 40-plate stacker.

A document outlining Assay Optimization for the EnVision is available for interested users.

Request Training Schedule Equipment

General Description

Multimode Plate Reader Capabilities

Absorbance (230-1100 nm)
Fluorescence (230-1100 nm)
Luminescence
Enhanced Luminescence
Time-Resolved Fluorescence
Fluorescence Polarization
DELFIA (TRF for heterogeneous assays)
LANCE (TR-FRET for homogeneous assays)
NanoBRET

EnVision 1

Located in SGM 611

This EnVision was purchased in 2021 with support from HMS Foundry

It is equipped with monochromators, enabling tunability of excitation and emission wavelengths for top absorbance and fluorescence intensity quantitation.
It has a dedicated laser (337 nm) for HTRF.
This EnVision has HTS 'Turbo' for AlphaScreen assays.

EnVision 2

Located in SGM 612

Provides HTS "Turbo" AlphaScreen. The purchase of this new model was supported in part by the Harvard Medical School Tools and Technologies Committee.

EnVision 3

Located in SGM 612

Filter Set	Wavelength (nm)	Bandwidth (nm)	Label	Type	Description	Bar Code
Monochromator*	585		X585co	Filter	Monochromator cutoff 585	142
Monochromator*	360		X360co	Filter	Monochromator cutoff 360	141
N/A	405	8		Filter	Photometric 405	302
N/A	n/a		BS50/BS50	Mirror	Dual beamsplitter enhanced	651
NanoBRET	585			Mirror	LUM/D585	673
	460	80	460/80	Filter	NanoBRET Blue	703
	647	75	647/75	Filter	BRET Deep Red 647	704
FITC FP Dual	505/535			Mirror	D505FP/D535	681
	480	30	X480	Filter	FITC FP 480	104
	535	40	M535p	Filter	FITC FP P-pol 535	209
	535	40	M535s	Filter	FITC FP S-pol 535	208
BODIPY TMR FP	555/595			Mirror	D555fp/D595	682
	595	60	M595p	Filter	Bodipy TMR P-pol	211
	595	60	M595s	Filter	Bodipy TMR S-pol	210
	531	25	X531	Filter	Bodipy TMR FP	106
LANCE LASER	407/630			Mirror	TRF Laser Lance	667
	407/505			Mirror	TRF Laser Lance	665
	615	8	M615a	Filter	LANCE Laser attenuated Europium filter	249
	665	7.5	M665	Filter	APC 665	205

* required for absorbance and fluorescence intensity top reads

Filters

Wavelength/ Bandpass (nm)	Label	Description	EnVision 2	EnVision 3	Bar Code
270/15		Custom 270/15	x		513
330/80		Custom 330	x	xx	501
340/60	P340	UV (TRF) 340	x		101
340/25	P340	Photometric 340	x	x	301
340/30		Custom 340	x	xx	502
355/40	X355	Umbelliferone 355	x	xx	103
380/30		Custom 380	x	x	503
405/8	P405	Photometric 405	xxx	xx	302
420/8	P420	Photometric 420	x	xx	308
460/25	M460	Umbelliferone 460	x	xx	207
470/40	D595	Custom GFP 470/40	x	xx	504
475/8	P475	Photometric 475	x	xx	309
480/30	X480	FITC FP 480	x	xx	104
485/14	X485	FITC 485	x	xx	102
495/10		Custom 495	x	xx	505
510/10	X510	YFP 510	xx	x	114
515/30	M515	BRET2 515	x	x	219
520/25		Custom 520	x	xx	506
525/50		Custom 525	x	xx	507
530/10	Mm530	YFP 530		x	221
531/25	X531	BODIPY TMR FP 531	xxx	xxxx	105
535/25	M535	FITC 535	xx	x	206
535/40	M535s	FITC FP S-POL (Em)	x	xx	208
535/40	M535p	FITC FP P-POL (Em)	x	x	209
550/9	P550	Photometric 550		x	312
570/100	M570w	Alphascreen 570		x	244
570/8	P570	Photometric 570	xx	x	318
580/10	M580	Emission 580	x	xx	233
580/40		Custom 580	x	x	508
590/20	M590	Rhodamine 590	x	xx	217
595/60	M595s	BODIPY TMR FP S-POL 595	x	x	210
595/60	M595p	BODIPY TMR FP P-POL 595	xx	x	211
595/60	M595w	Cy3 595	x	xxx	229
600/8	P600	Photometric 600	x	xx	319
615/8.5		Europium 615	x		203
620/10	X620	Cy5 620	x	x	118
640/40		Custom 640	x	x	509
650/8		Emission 650	x	xx	227
650/50		Custom 650	x	x	510
665/7.5	M665	APC 665	x	x	205
670/50		Custom 670/50		x	511
685/35		Cy5 685	xx	xx	243
715/20		Custom 715/20	x		512
720/8	P720	Photometric 720	x	x	322
n/a	LUM	Luminescence 700	x	x	212

Filters with the narrowest bandwidth are recommended for photometry. If additional filters with narrow bandwidths are required, please see screening facility staff.

Mirrors

Wavelength (nm)	Label	Description	EnVision 2	EnVision 3	Bar Code
400/630	D400/D630	TRF Dual	x		662
400	D400	LANCE/DELFIA	x	x	412
495	D495	Custom 495		xx	404
505	D505	FITC	x	x	403
505	D505FP	FITC FP Single		x	431
505/535	D505fp/D535	FITC FP dual	x	x	481
555/595	D555fp/D595	BODIPY TMR FP dual	x	x	682
555	D555	BODIPY TMR	xx	x	405
595	D595	Custom 595		x	404
640	D640as	Alphascreen		x	444
658	D658	Cy5	x	xx	420
700	Custom 700dcxr	Custom 700dcxr	x		404
n/a	BS50	Single beamsplitter		x	401
n/a	BS50/BS50	Dual beamsplitter	x	x	451
n/a	BS50/BS50	Dual beamsplitter enhanced	x	x	651

EnVision

Revvity EnVision plate reader

General Description

Multimode Plate Reader Capabilities

EnVision 1

EnVision 1

EnVision 2

EnVision 2

EnVision 3

EnVision 3

EnVision 1 Filters & Mirrors expand_more

EnVision 2 & 3 Filters expand_more

Filters

EnVision 2 & 3 Mirrors expand_more

Mirrors