# Synthego sgRNA Human Kinase This collection of [Synthego](https://www.synthego.com/products/crispr-kits/screening-libraries) synthetic single-guide RNAs (sgRNA) was purchased in 2019 and plated in January 2020. The design of this library is based on [Gencode Release 26 (GRCh38.p10)](https://www.gencodegenes.org/human/release_26.html). It incorporates recent advances in sgRNA algorithm design to decrease off-target effects and increase the probability of an indel resulting in gene knock out. The sgRNAs in this library are a discrete set that target kinases; the targets included in this library were determined by Synthego. Investigators will likely not screen both Synthego 1 and Synthego 2 libraries, as 780 of the targets in this library are also represented in Synthego 1. If you have questions about which library is most appropriate for your research goals, please contact [Jennifer Smith](mailto:jennifer_smith@hms.harvard.edu). SortNumber of sgRNA wells: 1,035 Plate Numbers: 51,519-51,522 (4 Plates) Concentration Information: 1 µM Control Information: - COPA (essential control) - KIF11 (essential control) - NLRP5 (non-essential control) - KRT77 (non-essential control) ![Plates 51519-51521](/sites/g/files/omnuum5406/files/iccb/files/plate_51519-51521.png) 384-Well Plate Format ![Plate 51522](/sites/g/files/omnuum5406/files/iccb/files/plate_51522.png) 384-Well Plate Format Additional vendor-provided information, including target and guide sequence, can be found in Screensaver. Jeff Bissen - Senior Account Specialist 612-750-6983 [jeff.bissen@synthego.com](mailto:/jeff.bissen@synthego.com)