# Relevant Supplies Many of the items listed in the tables below are available to purchase from the screening facility on an as needed basis. If you are an active ICCB-L screening user, please contact the [Screening Facility Staff](mailto:iccb_screen@listserv.med.harvard.edu) to request information on current pricing and availability of a particular item. ### Assay Plates expand\_more ## Sort384-well Plate Type Corning Cat. # Assay Volume (uL)Bottom TypeRecommended Assay Type(s)Clear, TC 3701 20-80 Clear, Polystyrene Absorbance Black, TC 3764 20-80 Clear, Polystyrene Imaging Black, S 3664 20-80 Clear, Poly-D-Lysine Imaging Black, S 3683 20-80 Clear, CellBind Imaging White, TC 3570 20-80 Opaque Luminescence Black, TC 3571 20-80 Opaque Luminescence ### Intermediate Assay Plates expand\_more Sort384-well Plate Type Eppendorf Cat. # Assay Volume (uL) Bottom Type twin.tec clear walls 12000-658 2-30 V bottom twin.tec yellow walls 12000-660 2-30 V bottom twin.tec red walls 12000-666 2-30 V bottom ### Manifolds and Aspiration Wands expand\_more SortInstrumentSupplierCat.#Item DescriptionImageThemo Combi Thermo-Fisher 24072670 8-channel Manifold, Standard-Bore, 5-2,500uL ![Combi manifold](/sites/g/files/omnuum5406/files/iccb/files/combicassette.png) 24073290 8-channel Manifold, Small-Bore, plastic tip 0.5-50uL 24073295 8-channel Manifold, Small-Bore, metall tip, 0.5-50uL Aspirator Wand Drummond 3-000-101 Stainless steel 24-channel. Manually aspirate volume from a 384-well assay plate ![Aspirator Wand](/sites/g/files/omnuum5406/files/iccb/files/dsc_0487.jpg) ### HTS Assay Kits expand\_more SortItem Description Cat. # Manufacturer Assay Type ATPLite 6016943 PerkinElmer Luminescent Cell Viability ADP-Glo (100,000 Assay) V9103 Promega Kinase Assay System BrightGlo (100mL) E2620 Luciferase Assay System BrightGlo (10 x 100mL) E2650 DualGlo (100mL) E2940 DualGlo (10 x 100mL) E2980 SteadyGlo (100mL) E2520 SteadyGlo (10 x 100mL) E2550 CellTiterGlo (100mL) G7572 Luminescent Cell Viability CellTiterGlo (10 x 100mL) G7573 ### Plate Seals expand\_more SortItem Type Cat. # Manufacturer Item Description AbsorbMax BK-50 Excel Scientific Black sealing film, non-sterile Breathable Assay Seal 241205 NUNC To seal assay plates during incubation to reduce evaporation Aluminum DMSO resistant 6570 Corning Used to manually seal assay or compound plates MicroClime Environmental Lid LL-0310 LabCyte Plate lid with chamber to cover assay plates during incubation to reduce evaporation Opaque Bottom Covers 6005189 PerkinElmer Black (apply to clear bottom plates to read in plate reader) 6005199 White (apply to clear bottom plates to read in plate reader) Temporary Assay Seal 19570 Qiagen To seal assay plates during incubation to reduce evaporation BrandTech Brand Self-adhesive 701371 BrandTech Vinyl material; to seal assay plates during storage or taking fluorescence measurements ### Library Storage expand\_more **RNAi Library Plates** Sort384-well Plate Type Cat. # Manufacturer Assay Volume (uL) Bottom Type sterile, RNAse/DNAse free, polypropylene 3656 Corning 95 round twin.tec blue walls 12000-664 Eppendorf 2-30 V bottom twin.tec green walls 12000-662 Eppendorf 2-30 V bottom ### Reagents and Tips expand\_more [Thermo Functional Genomics Quote for ICCB-L Investigators](/file_url/534). ICCB-L RNAi screeners interested in the current Dharmacon quote should contact [Jen Smith](mailto:jennifer_smith@hms.harvard.edu). SortItem Supplier Cat. # Item Description Nuclease-free water Qiagen 129114 10 bottles of 50mL each Nuclease-free water Qiagen 129115 1000mL bottle Nuclease-free water Qiagen 129117 Box of 5L 96-tip racks Agilent/ Fluotics 06880.002 200uL, non-sterile 96-tip racks Agilent/ Fluotics 08585.002 180uL, Sterile, Filtered 384-tip racks Agilent/ Fluotics 11484.002 30uL, non-sterile