Abstract
Auxin is a plant hormone that regulates many aspects of plant growth and development. We used a chemical genetics approach to identify SIR1, a regulator of many auxin-inducible genes. The sir1 mutant was resistant to sirtinol, a small molecule that activates many auxin-inducible genes and promotes auxin-related developmental phenotypes. SIR1 is predicted to encode a protein composed of a ubiquitin-activating enzyme E1-like domain and a Rhodanese-like domain homologous to that of prolyl isomerase. We suggest a molecular context for how the auxin signal is propagated to exert its biological effects.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Triphosphate / metabolism
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Amino Acid Sequence
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Arabidopsis / drug effects
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Arabidopsis / genetics
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Arabidopsis / growth & development
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Arabidopsis / metabolism*
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Arabidopsis Proteins / chemistry*
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism*
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Benzamides / metabolism
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Benzamides / pharmacology
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Binding Sites
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Gene Expression Profiling
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Gene Expression Regulation, Plant
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Genes, Plant
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Genes, Reporter
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Indoleacetic Acids / metabolism*
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Indoleacetic Acids / pharmacology
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Molecular Sequence Data
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Mutation
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Naphthols / metabolism
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Naphthols / pharmacology
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Oligonucleotide Array Sequence Analysis
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Phenotype
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Plant Leaves / drug effects
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Plant Leaves / growth & development
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Plant Roots / drug effects
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Plant Roots / growth & development
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Protein Structure, Tertiary
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Signal Transduction*
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Sirtuins / antagonists & inhibitors
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Transcription, Genetic
Substances
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Arabidopsis Proteins
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Benzamides
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Indoleacetic Acids
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Naphthols
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SIR1 protein, Arabidopsis
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sirtinol
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Adenosine Triphosphate
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Sirtuins