# Pin Transfer ![Pin Array](/sites/g/files/omnuum5406/files/styles/hwp_1_1__360x360_scale/public/iccb/files/pin-array-01.jpg?itok=F6DWE1mZ) ICCB-Longwood utilizes two different robotic platforms to conduct pin transfer of nanoliter volumes from compound storage plates into assay plates using stainless steel pin arrays (made by [V&P Scientific](http://vp-sci.com/)). - 96- and 384-well pin arrays - Transfer compounds into 96-, 384- and 1536-well microplates - 384-well assay plate volume 30 uL - calibrated to transfer 33 nL, 100 nL or 300 nL - 384-well assay plate volume 10 uL - calibrated to transfer 33 nL or 100 nL - The standard transfer volume for chemical screens in 384-well is 100 nL. - 96-well assay plate volume 150 uL - calibrated to transfer 200 nL, 250 nL or 300 nL [Pin Arrays & Calibration](/pin-arrays) provides more information on the pin arrays, the calibration process and selecting the appropriate pin array for your screen. ## K2 Compound Transfer Robot (SGM 611) ![Epson K2 pin transfer robot](/sites/g/files/omnuum5406/files/iccb/files/epson_k2-1.png) ![Epson K2 pin transfer robot](/sites/g/files/omnuum5406/files/iccb/files/epson_k2-2.png) - Custom pin transfer station featuring an Epson T6-602s SCARA robot designed and built at ICCB-Longwood - Paired with a Peak Robotics KX2 robot arm to deliver library and assay plates to and from the deck for transfer - Automated rotating CS6 carousel for microplate hotel racks - Custom Arduino-based relay board with 24V power supply to switch I/O devices - Automatic pin tool attachment/detachment K2 Specifications Pin Array Calibration Pin Array Wash ## K2 Specifications SortDesignRichard SiuConstruction Richard Siu & Alasdair Morton Integration Software Custom C# .Net Application developed by Richard Siu Robotic - Peak Robotics KX2-750 robot arm - Peak Robotics CS6 carousel - Epson T6-602s SCARA robot Table Custom Designed by SteelSentry Sonicator Sonicor SC-50 Methanol Bath Designed by ICCB-Longwood Team Dry Station Designed by ICCB-Longwood Team Pin Arrays Manufactured by [V&P Scientific](http://vp-sci.com/). - 33nL - 100nL - 300nL Cycle Time Approximately 1 minute per transfer, including wash and dry Plate Types Accepted 384-well, 96-well Total Plate Capacity 150 ## Pin Array Calibration #### The amount of compound transferred is dependent on several parameters - Pin shape and diameter - Volume of stock solution - Rate of pin withdrawal from the stock solution - Viscosity of liquid in the assay plate - Dwell time in the assay plate ## Pin Array Wash To prevent compound carryover, each pin array is washed after every library transfer. #### Pin Washing Steps: - PBS - 100% methanol flow-through wash - 20% methanol sonication step - (repeat twice) - Concludes with a 10-second high-pressure air dry ## Sonic Compound Transfer Robot (SGM 612) ![Sonic Compound Transfer Robot](/sites/g/files/omnuum5406/files/iccb/files/sonic_compound_transfer_robot.jpg) - Custom pin transfer station featuring an Epson T6-B602s SCARA robot designed and built at ICCB-Longwood - Paired with a Peak Robotics KX2-Plus robot arm to deliver library and assay plates to and from the deck for transfer - Automated rotating CS6 carousel for microplate sequential racks - Custom Arduino-based relay board with 24V power supply to switch I/O devices - Automatic pin tool attachment/detachment Sonic Specifications Pin Array Calibration Pin Array Wash ## Sonic Specifications SortDesignRichard SiuConstruction Richard Siu & Bryce Carr Integration Software Custom C# .Net Application developed by Richard Siu Robotics - Peak Robotics KX2-Plus-750 robot arm - Peak Robotics CS6 carousel - Epson T6-B602s SCARA robot Table Designed by Richard Siu, manufactured by Vention, assembled by Mitchell Kozakoff, Bryce Carr and Richard Siu Sonicator Cole Parmer Ultrasonic Cleaner Methanol Bath Designed by Stewart Rudnicki Dry Station Designed by Stewart Rudnicki Pin Arrays Manufactured by [V&P Scientific](http://vp-sci.com/). - 33nL - 100nL - 300nL Cycle Time Approximately 1 minute per transfer, including wash and dry Plate Types Accepted 384-well Total Plate Capacity ~190 (+/- 10 to 20 depending on plate heights) ## Pin Array Calibration #### The amount of compound transferred is dependent on several parameters - Pin shape and diameter - Volume of stock solution - Rate of pin withdrawal from the stock solution - Viscosity of liquid in the assay plate - Dwell time in the assay plate ## Pin Array Wash To prevent compound carryover, each pin array is washed after every library transfer. #### Pin Washing Steps: - PBS - 100% methanol flow-through wash - 20% methanol sonication step - (repeat twice) - Concludes with a 10-second high-pressure air dry