#  High-Content Microscopy Image Analysis 

 



The ICCB-Longwood High Content Screening Microscope Suite has four image analysis workstations available for viewing and analyzing the data from the high content screening microscopes, each with the MetaXpress software installed and 3 with IN Carta installed.

   ![MetaXpress image analysis workstation](/sites/g/files/omnuum5406/files/styles/hwp_1_1__720x720_scale/public/iccb/files/img_0218.jpg?itok=UiGWsHfp) 

 

## Software for High Content Screening

### Molecular Devices MetaXpress 

- MetaXpress enables users to select a standard image analysis routine or develop custom protocols to fit specific image analysis needs.
- Provides users with a range of tools to automate acquisition, processing, and analysis of cellular images
- Optional application-specific software modules for common assays are also available

###   
Molecular Devices IN Carta

- Create simple or complex analysis protocols tailored to experiment requirements
- Optional image pre-processing and object mask post-processing at each step
- Batch analysis of multiple experiments
- 3D analysis
- **SINAP**
    - Module with deep learning algorithms for segmenting objects
    - Useful for transmitted light, highly confluent, or low-signal images
    - Models are iteratively trained by scientist drawing over images
- **Phenoglyphs**
    - Analyzes hundreds of cellular features simultaneously to create a comprehensive phenotypic profile and resolve subtle phenotypic changes
    - Classification is confirmed or corrected by scientist until predictions are correct

For more information on MetaXpress and IN Carta, please contact the [ICCB-L Screening Room Team](mailto:iccb_screen@listserv.med.harvard.edu) or view the [Molecular Devices website.](https://www.moleculardevices.com/)

The Image Analysis Collaboratory (IAC) at Harvard Medical School is another option for image analysis guidance and support. They provide comprehensive support and training for researchers working with biological imaging data. Visit [iac.hms.harvard.edu](http://iac.hms.harvard.edu/) to learn more.



 

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###    Available MetaXpress Analysis Tools  expand\_more  

 

- Angiogenesis Tube Formation
- Cell Scoring
- Cell Health
- Custom Module Editor ([CME](https://www.moleculardevices.com/en/assets/app-note/dd/img/custom-module-editor-for-metaxpress-high-content-image#gref))
- Count Nuclei
- Granularity
- Live/Dead
- Micronuclei
- Mitotic Index
- Multi Wavelength Cell Scoring
- Neurite Outgrowth
- Transfluor
- Translocation
- Translocation-Enhanced