Human 14 - Ubiquitin Conjugating Enzymes #1

This collection of Dharmacon siGENOME siRNAs was purchased in 2014 as part of the RNAi Global Consortium with funding from Harvard Medical School’s Tools and Technologies Committee. The design of this library is based on RefSeq version 27 and incorporates recent advances in siRNA algorithm design to help decrease RNAi off-target effects. The siRNAs are arrayed as SMARTpools.

Number of siRNA wells 89
Plate Numbers 51,220 (1 Plate) 
Vendor Library Catalog Number G-005615
Concentration Information 1 µM
Control Information
  • Non-Targeting siRNA #2 (D-001210-02)
  • Non-Targeting siRNA #3 (D-001210-03)
  • Non-Targeting siRNA #5 (D-001210-05)
  • PLK1 SMARTpool (M-003290-01)
  • KIF11 SMART pool (M-003317-01)
  • siGLO RISC-free siRNA (D-001600-01)

384-Well Plate Formats

The SMARTpools in this library are a discrete set referred to as ubiquitin conjugating enzymes #1 that target cullins, as well as E1, E2 and HECT E3 ubiquitin ligases. The targets included in this library were determined by Dharmacon. There are eight focused libraries, represented by Dharmacon 2014 Human 9 through Dharmacon 2014 Human 16 libraries. The SMARTpools in this library and the other focused libraries are also plated continuously in Dharmacon 2014 Human 6 Druggable Genome Compiled 9-16 Focused Libraries. Investigators should not screen both the Human 9 – 16 libraries and Human 6 library. If you have questions about which library is most appropriate for your research goals, please contact Jennifer Smith.

Dharmacon is a subsidiary of Horizon Discovery and was formerly part of GE Healthcare.