#  ICCB-Longwood Mechanism of Action Library 10mM 

 



The ICCB-Longwood Mechanism of Action Library (ICCB-L MoA) is a growing collection of well-annotated, high quality compounds with known biological targets. A tool score, described by Wang and colleagues ([Cell Chemical Biology 23, 862-874](https://www.ncbi.nlm.nih.gov/pubmed/27427232)), was utilized to identify the best target-compound pairs based upon target specificity and potency, with Rank 1 being the highest. This collection, when complete, will also include Rank 2 and Rank 3 compounds for some biological targets.

The curation of this library was made possible through the support of [HMS LINCS](http://lincs.hms.harvard.edu/) and guidance from Yuan Wang and Jeremy Jenkins at Novartis Institutes for BioMedical Research. Compounds were purchased by ICCB-Longwood and the [Assay Development & Screening Facility](https://www.childrenshospital.org/node/152965/cores/assay-development-and-screening-facility) at Boston Children's Hospital, and funded in part by a grant to BCH from [Massachusetts Life Sciences Center](http://www.masslifesciences.com/). Harvard Medical School's [Foundry Award Program](https://foundryawards.hms.harvard.edu/) also provided funds for the acquisition of this library.

Note that two wells contain fluorescein (3744:C17 and 3752:I07), a highly fluorescent dye with a bright yellowish-green emission that may produce false results in fluorescence and absorbance assays.

Each compound is plated at 10 mM in Quadrant A1 of the 384-well plate. This library is primarily utilized by investigators screening in 96-well format. Please see map below for a visual layout.

Plates 3740-3744 were plated in October 2017 and contain 400 Rank 1 compounds. Plates 3745-3748 were plated in February 2018 and contain 320 Rank 1 and 2 compounds. Plates 3749 and 3750 were plated in September 2018 and contain Rank 1, 2 and 3 compounds. Plates 3751-3755 were plated in October 2020 and contain Rank 1, 2 and 3 compounds.

SortNumber of Compounds:

 1245

Plate Numbers:

3740-3755 (16 Plates)

Concentration Information:

10mM in DMSO





 ![Plates 3740-3754](/sites/g/files/omnuum5406/files/iccb/files/plate_3740-3754.png)

 

384-Well Plate Format ![Plate 3755](/sites/g/files/omnuum5406/files/iccb/files/plate_3755.png)

 

384-Well Plate Format

 

This library requires permission before screening. ICCB-Longwood screeners interested in screening this library should contact [Jennifer Smith](mailto:jennifer_smith@hms.harvard.edu) for more details.