#  EnVision 

 



## Revvity EnVision plate reader

ICCB-Longwood has three EnVision plate readers that can be run in single plate mode or with a detachable 40-plate stacker.

A document outlining [Assay Optimization](/file_url/347) for the EnVision is available for interested users.

 ![EnVision 3 plate reader](/sites/g/files/omnuum5406/files/iccb/files/envision.png)

 



 

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 General Description EnVision 1 EnVision 2 EnVision 3 

## General Description

 

 

##  Multimode Plate Reader Capabilities

- Absorbance (230-1100 nm)
- Fluorescence (230-1100 nm)
- Luminescence
- Enhanced Luminescence
- Time-Resolved Fluorescence
- Fluorescence Polarization
- DELFIA (TRF for heterogeneous assays)
- LANCE (TR-FRET for homogeneous assays)
- NanoBRET



 



 

 

 

## EnVision 1

 

 

### EnVision 1

Located in SGM 611

This EnVision was purchased in 2021 with support from HMS Foundry

- It is equipped with monochromators, enabling tunability of excitation and emission wavelengths for top absorbance and fluorescence intensity quantitation.
- It has a dedicated laser (337 nm) for HTRF.
- This EnVision has HTS 'Turbo' for AlphaScreen assays.

 ![EnVision 1](/sites/g/files/omnuum5406/files/iccb/files/envision1_iccbl.jpg)

 



 



 

 

 

## EnVision 2

 

 

### EnVision 2

Located in SGM 612

Provides HTS "Turbo" AlphaScreen. The purchase of this new model was supported in part by the Harvard Medical School Tools and Technologies Committee.

 ![EnVision 2 plate reader](/sites/g/files/omnuum5406/files/iccb/files/envision2.jpg)

 



 



 

 

 

## EnVision 3

 

 

### EnVision 3

Located in SGM 612

 ![EnVision 3 plate reader](/sites/g/files/omnuum5406/files/iccb/files/envision.png)

 



 



 

 

 

 

 



###    EnVision 1 Filters & Mirrors  expand\_more  

 

SortFilter Set Wavelength (nm)  Bandwidth (nm) LabelTypeDescription Bar Code Monochromator*

585

 

X585co

Filter

Monochromator cutoff 585

142

360

 

X360co

Filter

Monochromator cutoff 360

141

N/A

405

8

 

Filter

Photometric 405

302

N/A

n/a

 

BS50/BS50

Mirror

Dual beamsplitter enhanced

651

NanoBRET

585

 

 

Mirror

LUM/D585

673

460

80

460/80

Filter

NanoBRET Blue

703

647

75

647/75

Filter

BRET Deep Red 647

704

FITC FP Dual

505/535

 

 

Mirror

D505FP/D535

681

480

30

X480

Filter

FITC FP 480

104

535

40

M535p

Filter

FITC FP P-pol 535

209

535

40

M535s

Filter

FITC FP S-pol 535

208

BODIPY TMR FP

555/595

 

 

Mirror

D555fp/D595

682

595

60

M595p

Filter

Bodipy TMR P-pol

211

595

60

M595s

Filter

Bodipy TMR S-pol

210

531

25

X531

Filter

Bodipy TMR FP

106

LANCE LASER

407/630

 

 

Mirror

TRF Laser Lance

667

407/505

 

 

Mirror

TRF Laser Lance

665

615

8

M615a

Filter

LANCE Laser attenuated Europium filter

249

665

7.5

M665

Filter

APC 665

205





\* *required for absorbance and fluorescence intensity top reads*



 

 

 



###    EnVision 2 & 3 Filters  expand\_more  

 

## Filters

SortWavelength/  
Bandpass (nm)LabelDescriptionEnVision  
2EnVision  
3Bar Code270/15

 

Custom 270/15

x

 

513

330/80

 

Custom 330

x

xx

501

340/60

P340

UV (TRF) 340

x

 

101

340/25

P340

Photometric 340

x

x

301

340/30

 

Custom 340

x

xx

502

355/40

X355

Umbelliferone 355

x

xx

103

380/30

 

Custom 380

x

x

503

405/8

P405

Photometric 405

xxx

xx

302

420/8

P420

Photometric 420

x

xx

308

460/25

M460

Umbelliferone 460

x

xx

207

470/40

D595

Custom GFP 470/40

x

xx

504

475/8

P475

Photometric 475

x

xx

309

480/30

X480

FITC FP 480

x

xx

104

485/14

X485

FITC 485

x

xx

102

495/10

 

Custom 495

x

xx

505

510/10

X510

YFP 510

xx

x

114

515/30

M515

BRET2 515

x

x

219

520/25

 

Custom 520

x

xx

506

525/50

 

Custom 525

x

xx

507

530/10

Mm530

YFP 530

 

x

221

531/25

X531

BODIPY TMR FP 531

xxx

xxxx

105

535/25

M535

FITC 535

xx

x

206

535/40

M535s

FITC FP S-POL (Em)

x

xx

208

535/40

M535p

FITC FP P-POL (Em)

x

x

209

550/9

P550

Photometric 550

 

x

312

570/100

M570w

Alphascreen 570

 

x

244

570/8

P570

Photometric 570

xx

x

318

580/10

M580

Emission 580

x

xx

233

580/40

 

Custom 580

x

x

508

590/20

M590

Rhodamine 590

x

xx

217

595/60

M595s

BODIPY TMR FP S-POL 595

x

x

210

595/60

M595p

BODIPY TMR FP P-POL 595

xx

x

211

595/60

M595w

Cy3 595

x

xxx

229

600/8

P600

Photometric 600

x

xx

319

615/8.5

 

Europium 615

x

 

203

620/10

X620

Cy5 620

x

x

118

640/40

 

Custom 640

x

x

509

650/8

 

Emission 650

x

xx

227

650/50

 

Custom 650

x

x

510

665/7.5

M665

APC 665

x

x

205

670/50

 

Custom 670/50

 

x

511

685/35

 

Cy5 685

xx

xx

243

715/20

 

Custom 715/20

x

 

512

720/8

P720

Photometric 720

x

x

322

n/a

LUM

Luminescence 700

x

x

212





*Filters with the narrowest bandwidth are recommended for photometry. If additional filters with narrow bandwidths are required, please see screening facility staff.*



 

 

 



###    EnVision 2 & 3 Mirrors  expand\_more  

 

## Mirrors

SortWavelength (nm)LabelDescriptionEnVision 2EnVision 3Bar Code400/630

D400/D630

TRF Dual

x

 

662

400

D400

LANCE/DELFIA

 x

x

412

495

D495

Custom 495

 

xx

404

505

D505

FITC

x

x

403

505

D505FP

FITC FP Single

 

x

431

505/535

D505fp/D535

FITC FP dual

x

x

481

555/595

D555fp/D595

BODIPY TMR FP dual

x

 x

682

555

D555

BODIPY TMR

xx

x

405

595

D595

Custom 595

 

x

404

640

D640as

Alphascreen

 

x

444

658

D658

Cy5

x

 xx

420

700

Custom 700dcxr

Custom 700dcxr

x

 

404

n/a

BS50

Single beamsplitter

 

x

401

n/a

BS50/BS50

Dual beamsplitter

x

x

451

n/a

BS50/BS50

Dual beamsplitter enhanced

 x

x

651