Automated Pipettors

ICCB-Longwood utilizes several different pipettor based liquid handling instruments. Each of these is versatile in its capabilities and designed for precision pipetting.   

Agilent Bravo

Agilent Bravo

The Agilent Bravo is a versatile liquid handler enclosed in a Baker Bioprotect II hood. This instrument has an open air flow design and both 96- and 384-channel disposable tip pipette heads, with volume ranges from 0.5 to 200 uL. It is primarily used for siRNA liquid handling, including both screening and library reformatting.

This instrument is operated by ICCB-Longwood Screening Facility staff members.

Tecan Evo75

Tecan Evo75

The “Hit Picker” at the ICCB-L Screening Facility is comprised of two instruments, the Tecan EVO75 Liquid Handling Platform (left), and a Velocity11 2R BenchCel Stacker (right) integrated together using Visual Basic programming. The EVO75 has 2 independent disposable tips (DiTis) and is capable of picking a maximum of 300 wells per hour. A bar code reader has been incorporated into the system for tracking plates and real-time error detection. The EVO75 can accommodate both 96- and 384-well plates as well as 1.5 mL microfuge tubes, and volume ranges from 1 uL to 250 uL. A comma separated (.csv) input file is used as the source for the picks.

This instrument is operated by ICCB-Longwood Screening Facility staff members.