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To read more information about the FluoroPoint single molecule detection assays, click here. (PDF)
ICCBL has an Olympus Fluoropoint single molecule fluorescence detection system on demo until approximately March 2008. The capabilities of this instrument are described below. For more detailed information about this instrument, please click here to see a PowerPoint presentation from Olympus. If you are interested in trying out this instrument with your assay, please contact the following people to arrange a time:
Nate Moerke, Ph.D. - Senior Assay Developer, ICCBL
nathanmoerke"AT"gmail.com, 617-432-8582
or
Stewart Rudnicki - Screening Facility Manager, ICCBL
stewart_rudnicki"AT"hms.harvard.edu, 617-432-5815
This instrument takes measurements at the single molecule level and can give you information about bio-molecule interaction. Examples include Protein-Protein or Protein-DNA binding, but are not limited to these. It uses 3 analysis methods:
- FCS (Fluorescence Correlation Spectroscopy) – Measures size of molecules, concentration of each molecule, and number of molecule types in the solution
- FIDA (Fluorescence Intensity Distribution Analysis) – Brightness of each molecule, Concentration of each molecule, number of molecule types in the solution.
- FIDA-PO (FIDA Polarization) – FP analysis at the molecular level, concentration of each molecule, numbers of molecule type in the solution.
Specific uses of the 3 analysis methods above are listed below:
- Monitoring of molecule size – looking for aggregate formation (dimers, trimers)
- Determining the concentration and number of molecule types in solution
- Screening protease inhibitors
- SNP Typing (coming soon)
- Antibody-Antigen reactions
- Detecting Ligand-Receptor interactions
- Fluorescence Polarization – Molecule interactions and binding
- Enzyme Reactions
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