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Note: The EnVision is a very sensitive plate reader and it has been demonstrated that it is not optimal for use with Alamar Blue Assays.
The 40-plate stacker Perkin Elmer EnVision plate
reader is capable of standard multi-mode plate reader capabilities:
- Absorbance
- Fluorescence
- Luminescence
- Enhanced Luminescence
- Time-Resolved Fluorescence
- Fluorescence
Polarization
- DELFIA® (TRF for heterogenous assays)
- LANCE® ( TR-FRET for homogenous assays)
Certain filters and mirrors are designed for FP assays. These are indicated in the tables below.
Envision 1 - Upgraded
Envision 2
Envision 3
Envision 1 - Upgraded
Note: As of 4/5/07 Envision1 was completed with an upgrade to include Alphascreen (PDF) and Ultra Luminescence. If you have any questions regarding these new capabilities please see a member of the screening room staff.
Excitation:
| Filter Name |
Wavelength (nm) |
Bandpass (nm) |
FP |
| Photometric 330 |
330 |
80 |
|
| Photometric 340 |
340 |
25 |
|
| Umbelliferone |
355 |
40 |
|
| Photometric 405 |
405 |
8 |
|
| Photometric 420 |
420 |
8 |
|
| FITC FP |
480 |
30 |
x |
| FITC |
485 |
14 |
|
| BODIPY TMR FP |
531 |
25 |
x |
| Photometric 570 |
570 |
8 |
|
| Photometric 600 |
600 |
8 |
|
| Cy5 |
620 |
10 |
|
| Photometric 720 |
720 |
8 |
|
Emission:
Filter Name |
Wavelength (nm) |
Bandpass (nm) |
FP |
| Umbelliferone |
460 |
25 |
|
| BRET2 |
515 |
30 |
|
| FITC FP S-pol |
535 |
40 |
x |
| FITC FP P-pol |
535 |
40 |
x |
| FITC |
535 |
25 |
|
| AlphaScreen |
570 |
100 |
|
| Rhodamine |
590 |
20 |
|
| Cy3 |
595 |
60 |
|
| Custom Emission |
650 |
8 |
|
| APC |
665 |
7.5 |
|
| Luminescence |
700 |
--- |
|
Dichroic Mirrors:
Filter Name |
Wavelength (nm) |
Test Compatibility |
| LANCE/DELFIA |
400 |
|
| FITC |
505 |
|
| FITC FP Dual |
D505fp/D535 |
x |
| BODIPY TMR |
555 |
|
| AlphaScreen |
640 |
|
| Cy5 |
658 |
|
| 50/50 Beamsplitter |
--- |
|
| Luminescence Mirror |
--- |
|
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Envision 2
Excitation:
Filter Name |
Wavelength (nm) |
Bandpass (nm) |
FP |
| Photometric 340 |
340 |
25 |
|
| Umbelliferone |
355 |
40 |
|
| Photometric 405 |
405 |
8 |
|
| Photometric 420 |
420 |
8 |
|
| Custom |
470 |
40 |
|
| FITC FP |
480 |
30 |
x |
| FITC |
485 |
14 |
|
| YFP |
510 |
10 |
|
| Custom |
525 |
50 |
|
| BODIPY TMR FP |
531 |
25 |
x |
| Custom |
580 |
8 |
|
| Photometric 600 |
600 |
8 |
|
| Cy5 |
620 |
10 |
|
| Custom |
640 |
40 |
|
| Photometric 720 |
720 |
8 |
|
Emission:
Filter Name |
Wavelength (nm) |
Bandpass (nm) |
FP |
| Umbelliferone |
460 |
25 |
|
| YFP |
530 |
10 |
|
| FITC |
535 |
25 |
|
| FITC FP S-pol |
535 |
40 |
x |
| FITC FP P-pol |
535 |
40 |
x |
| custom |
570 |
8 |
|
| Rhodamine |
590 |
20 |
|
| Cy3 |
595 |
60 |
|
| Emission 650 |
650 |
8 |
|
| APC |
665 |
7.5 |
|
| Luminescence |
700 |
--- |
|
Dichroic Mirrors:
Filter Name |
Wavelength (nm) |
FP |
| LANCE® / DELFIA® |
400 |
|
| FITC |
D505 |
|
| FITC FP Dual |
D505fp/D535 |
x |
| BODIPY TMR |
D555 |
|
| 50/50 Beamsplitter |
--- |
|
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Envision 3
Excitation:
Filter Name |
Wavelength (nm) |
Bandpass (nm) |
FP |
| Umbelliferone |
355 |
40 |
|
| Photometric 405 |
405 |
8 |
|
| Photometric 420 |
420 |
8 |
|
| FITC FP |
480 |
30 |
x |
| FITC |
485 |
14 |
|
| BODIPY TMR FP |
531 |
25 |
x |
| Cy5 |
620 |
10 |
|
Emission:
Filter Name |
Wavelength (nm) |
Bandpass (nm) |
FP |
| Umbelliferone |
460 |
25 |
|
| FITC |
535 |
25 |
|
| FITC FP S-pol |
535 |
40 |
x |
| FITC FP P-pol |
535 |
40 |
x |
| Cy3 |
595 |
60 |
|
| Photometric 650 |
650 |
8 |
|
Dichroic Mirrors:
Filter Name |
Wavelength (nm) |
FP |
| LANCE® / DELFIA® |
400 |
|
| FITC |
505 |
|
| FITC FP Dual |
D505fp/D535 |
x |
| BODIPY TMR |
D555 |
|
| 50/50 Beamsplitter |
--- |
|
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Filters with the narrowest bandwidth are recommended
for photometry. If additional filters with narrow bandwidths are
required, please see screening facility staff.
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