Seiko Compound Transfer Robot
The ICCB-Longwood currently utilizes three different instruments to conduct pin transfer
operations. Each is used to transfer nanoliter volumes from compound storage
plates into assay plates using stainless steel pin arrays (made by V&P
Scientific).
The Seiko is a cartesian robot that was custom designed and built
at the ICCB-Longwood. This system is highly robust and can rapidly process
many samples without requiring costly disposable tips. The steel
pins are washed, sonicated in a water/methanol mix and dried with
pressurized air. The robot is shown above. Click
here for more details about compound
transfer using this robot.
In addition the Seiko has been integrated with a TwisterII robotic arm from Caliper. Click on the link below to see a brief video of the Seiko working with the TwisterII.
Click here to view robots in action
(900KB QuickTime movie file).
Epson Compound Transfer Robot
The Epson compound transfer robot, is a custom design similar to the Seiko robot setup. The pin arrays are calibrated to transfer 100nL and are then washed with a flow through methanol cycle as well as an air dry station. A sonicating bath will be added in the future to more closely mimic the wash cycle of the Seiko robot.
Pin Arrays
We have both 96- and 384-well pin arrays. The pin arrays allow transfer of 300nl, 100 nl, or 10-20 nl volumes, depending on pin diameter. Click here for more information about pin arrays and selecting a pin array for your screen.
CyBio CyBi-Well vario System
In addition to being a low-speed, high accuracy liquid handling
device (click
here for details), the CyBio CyBi-Well vario was upgraded
to perform pin-transfer operations. Currently, 10-20nl and 100nl
pin arrays are available. Calibrations performed by the staff indicate
that the 100nl pin array transfers virtually the same quantity
as that of the Seiko pin-transfer robot’s
100nl pin array. The CyBi-Well vario, like the Seiko, also washes
with methanol; however, the pins are then blotted and air-dried at
the end of the wash cycle.
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