 | | Screening Supplies |
384-Well Plates Liquid Handling Supplies
Imaging Plate Reader Supplies
Tissue Culture Supplies
Assay Kits
Plate Seals
Other Screening Supplies
RNAi Specific Supplies
384-Well Plates
Standard Volume 384-well Assay Plates (working volume 20-80uL)
| Clear bottom assay plates (Polystyrene base) |
| Item |
Color |
Type 1 |
Supplier |
Purchase Number |
| Corning |
black |
TC |
Westnet |
3712 |
| Opaque bottom assay plates | | Item | Color | Type 1 | Supplier | Purchase Number |
| Corning |
white |
TC |
Westnet |
3570 (formerly 3704) |
| Corning |
white |
NS |
Westnet |
3572 (formerly 3705) |
| Corning |
black |
TC |
Westnet |
3571 (formerly 3709) |
| Corning |
black |
NS |
Westnet |
3573 (formerly 3710) |
| Corning | clear | TC | Westnet |
3701 |
| Corning | clear | NS | Westnet |
3702 |
Low Volume 384-well Assay Plates (working volume 5-40uL)
| Opaque bottom assay plates | | Item |
Color |
Type 1 |
Supplier |
Purchase Number |
| Corning |
black |
NS, NBS |
Westnet |
3820 |
| Corning |
black |
NS |
Westnet |
3821 |
| Corning |
White |
NS, NBS |
Westnet |
3673 |
| Corning |
White |
NS |
Westnet |
3674 |
Compound Stock Plates
| Polypropylene plates |
| Item |
Volume |
Type |
Supplier |
Purchase Number |
| Nunc 96-well |
500ul |
round bottom |
Fisher |
12-565-368 |
| VWR 96 deep well |
1mL |
round bottom |
VWR |
40002-011 |
| Genetix384-Well |
50uL |
“V” Bottom |
Genetix |
X5005 |
| Marsh384-Well |
120uL |
Conical Bottom |
ABgene |
AB-0781 |
| ABgene384-Well |
55uL |
"V" Bottom |
ABgene |
AB-1056 |
Notes:
1 Types of plates: TC = tissue culture with lid; NS = not sterile, without lid, NBS=non-binding surface
2 Most Commonly Used Plates
3 Plates used for monitoring growth of yeast and bacteria
4 Please note that the LJL low-volume plates are not compatible with the Twister II robotic arm
Liquid Handling Supplies Supplies for Bio-Tek Precision 2000
| Ordering for BIO-TEK Precision 2000 | | Service Supplier | Address | Contact | | Bio-Tek Instruments Inc. | Highland Park, Box 998
Winooski, VT 05404-0998 | Tel: (888) 451-5171
E-mail: sales@BioTek.com |
| Item | Catalogue Number | | 8-channel dispense manifold |
7110515s |
DMSO Resistant tubing for the manifold
(standard tubing comes with each manifold). | Contact Bio-Tek |
| Rainin 250 pipette Tips (250uL) |
RT-250 |
| Autoclavable check valves |
Contact customer services at 1-800-451-4540; ext: 211 Purchase #: 68083 |
Supplies for Bio -Tek MicroFill | Ordering for BIO-TEK MicroFill | | Service Supplier | Address | Contact | | Bio-Tek Instruments Inc. | Highland Park, Box 998
Winooski, VT 05404-0998 | Tel: (888) 451-5171
E-mail: sales@BioTek.com |
| Item |
Catalogue Number |
| 16-channel autoclavable manifold |
7140508S
| | Autoclavable tubing |
7140510 |
| Autoclavable syringe pump |
7140516 |
| Autoclavable check valves |
Contact customer services at 1-800-451-4540; ext: 211 Purchase #: 68083 |
Supplies for Matrix WellMate Ordering for Matrix WellMate | | Service Supplier | Address | Contact | | Matrix | 22 Friars Dr.Hudson, NH 03051 |
Tel: (800)345-0206 |
| Item | Catalogue Number | | 8-channel, Standard-Bore Disposable Tubing Cartridge. Pre-sterilized. Features a 0.023" (0.58mm) inner diameter nozzle orifice. Dispenses 20ul to2000ul (used for 96-well assay plates). |
201-30001
| | 8-channel, Small-Bore Disposable Tubing Cartridge. Pre-sterilized. Features a0.015" (0.38mm) inner diameter nozzle orifice. Dispenses 2ul to 200ul (used for 384-well plates). |
201-30002 |
Pipette Tips for Liquid Handling
| Pipette Tip Suppliers | | Name |
Address |
Contact Information |
| Bio-Tek Instruments, Inc. |
Highland Park, Box 998
Winooski, VT 05404-0998 |
Tel: (888) 451-5171 Email:sales@BioTek.com |
| CyBio |
500 West Cummings Park Suite 1200
Woburn, MA 01801 |
Tel: (877) 879-5624 |
| Matrix |
22 Friars Dr.
Hudson, NH 03051 |
Tel: (800)345-0206 |
| Velocity11 |
3565 Haven Avenue
Menlo Park, CA 94025
|
Tel: (650)846-6600
Email: orders@velocity11.com |
| Pipette Tips for Liquid Handling | | Item |
Supplier |
Order Number |
| Velocity11 tips |
Velocity11 |
see V11 webpage for ordering information |
Bio-Tek tips 10,
racks of 96 | Bio-Tek Instruments Inc. |
RT-250 |
| CyBio96 Tips | CyBio |
see CyBio webpage for ordering information |
Matrix Tips (for CyBio)
20, magazines of 96 | Matrix |
5517 |
Thermo Multidrop Micro
Service Supplier | | Name |
Address |
Contact |
| Thermo Electron |
450 Fortune Blvd.
Milford , MA 01757 | Tel: (866) 984-3766 |
| SciCal |
622 West Chatham StApex, NC 27502 |
Tel: (800)892-9817
E-mail: scical@scical.com |
| Item | Catalogue Number | | 8-channel metal tip dispensing cassette | 24073295 | | 8-channel moulded-tip dispensing cassette | 24073290 | Multidrop Cassette Assemblies and Calibration Multidrop cassette assemblies can be ordered from and calibrated by Thermo Labsystems or SciCal.
| Service Supplier | | Name | Address | Contact | | Thermo Labsystems |
8 East Forge Parkway
Franklin, MA 02038 | Tel: (508)520-0009
labsystems.us.thermobio.com | | SciCal |
622 West Chatham StApex, NC 27502 |
Tel: (800)892-9817
E-mail: scical@scical.com |
| SciCal Services | | Rebuilding of tubing cassettes | | Tubing kit | | Rebuild/calibrate tubing cassette | | Calibration | | New cassette assemblies |
Imaging Plate Reader Supplies
| Clear Bottom Assay Plates | | Item | Color | Type | Supplier | Purchase Number | | Corning Costar 384-well |
Black w/ Clear bottom |
TC |
Westnet |
3712 |
| Corning Costar 96-well |
Black w/Clear bottom |
TC |
Westnet |
3603 |
Tissue Culture Supplies | Supplier Information | | Name | Address | Contact Information | | Gibco | Gibco Life Technology/Invitrogen
3175 Staley Rd.
Grand Island NY 14072 | Tel: (800) 955-6288
Fax: (800) 331-2286 |
| Antifungal/Antibiotic Mixture for Tissue Culture | | Item | Catalogue Number | Antibiotic/Anti-Mycotic 100x
solution contains Penicillin G,
Streptomycin and Amphotercin B as Fungaizone. | Gibco #15240-062 |
Assay Kits | Assay Suppliers | | Name | Address | Contact Information | | PerkinElmer Life Sciences | 549 Albany St.
Boston, MA 02118
http://las.perkinelmer.com | Tel: 1 (800) 762-4000
Fax: (203) 944-4904
Email:productinfo@perkinelmer.com
| | Promega | 2800 Woods Hollow Rd.
Madison, WI 53711-5399 | Tel: (800) 356-9526
Fax: (800) 356-1970 | | Molecular Probes | P.O. Box 22010
Eugene, OR 97402-0469 | Tel: (800) 438-2209 |
| Cell Viability Assays | | Item |
Supplier |
Order Number |
| ATPLite |
PerkinElmer |
6016943 |
| CellTiterGlo-10x100ml |
Promega |
G7573 |
| CellTiterGlo-100ml |
Promega |
G7572 |
| CellTiterGlo-10x10ml |
Promega |
G7571 |
| CellTiterGlo-10ml |
Promega |
G7570 |
| ATPase Activity Assay | | ATPase-ATP assay | Molecular Probes | A-6608 |
Plate Seals Temporary plate seals These covers are not DMSO-resistant; they are 3M mailing labels! However, they can serve as seals while working with your assay plates to help prevent evaporation. Available from Qiagen as Catalog # 19570. They can also be ordered from Staples as Catalog # MMM822P, but you will have to cut them down to the 4.5" X 3" size. | Temporary Plate Seal Suppliers | | Name | Address | Contact Information | | Qiagen | 28159 Avenue Stanford
Valencia, CA 91355 | Tel: (800) 426-8157
Fax: (800) 718-2056
http://www.qiagen.com/ | | Staples | 401 Park Drive
Boston, Ma 02115 | Tel: (617) 638-3292
Fax: (800) 356-1970 |
Storage quality (DMSO resistant) plate seals
These Corning/Costar seals (Costar #6570) were once used by the screening room staff to seal the compound stock plates. They can also be used on assay plates. | Supplier | Address | Contact Information | Item Number | | Fisher Scientific | Pittsburg, PA 15275 | Tel: (800) 766-7000
Fax: (800) 926-1166 | 072-00684 |
Other Screening Supplies
Instant Sealing Sterilization Pouches - "Autoclave Bags"
These pouches are large enought to fit a Matrix Wellmate or BioTek MicroFill Manifold into for being sterilized in an autoclave. These are available from Fisher Scientific at fishersci.com.
7 x 13" 2mL-thick blue transparent polyester/polypropylene pouch:
Catalog # 01-812-55
 top of page
Aspirator Tool - “The Wand”
The Wand is a 24-channel adaptor for aspirating liquid from 384-well assay plates.
A polycarbonate wand is available from V & P Scientific as Catalog # VP 186L.
V & P Scientific
Phone: (800) 455-0644
E-mail: sales@VP-Scientific.com
The Drummond Scientific stainless steel wand is availble from VWR,
24-channel Catalog # 3-000-101.
Web: http://www.vwrsp.com/
Opaque Bottom Covers for clear bottom assay plates
To apply to clear bottom plates assay plates so that they can be read in a plate reader.
Available from PerkinElmer
Black stickers: Catalog # 6005189 55 stickers
White stickers: Catalog # 6005199 55 stickers Perkin Elmer Life Sciences
http://las.perkinelmer.com
Tel: 1 (800) 762-4000
Fax: (203) 944-4904
Email: productinfo@perkinelmer.com
Labels for chemical stock plates Microtube Tough-Tags for laser & inkjet printers. Pre-cut, peel-off. Available from Marsh as Cat. # TT15-LAS. Marsh Biomedical Products Inc
565 Blossum Road
Rochester, NY 14610
Tel: (716) 654.4800
Fax: (716) 654.4810
RNAi Specific Supplies
Assay Plates
384-well assay plates for RNAi transfections are also the plates listed above for other types of assays. Please click here to see these plate types and ordering information.
Intermediate Assay Plates
Eppendorf twin.tec 384-well plates |
| Description |
Ordering Information |
Quantity |
Supplier |
| Clear |
VWR#: 1200-658; Eppendorf#: 951020702 |
25 |
VWR |
| Red |
VWR#: 1200-666; Eppendorf#: 951020745 |
25 |
VWR |
| Yellow |
VWR#: 1200-660; Eppendorf#: 951020711 |
25 |
VWR |
Library Plates
384-well Library Plate Types |
| Description |
Ordering Information |
Quantity |
Supplier |
| Corning Sterile, RNAse/DNase free, Round Bottom, Polypropylene, 95uL plate |
3656 |
25 |
Westnet
(781)828-7772 |
| Eppendorf twin.tec Blue |
VWR#: 1200-664;
Eppendorf#: 951020737
|
25 |
VWR |
| Eppendorf twin.tec Green |
VWR#: 1200-662;
Eppendorf#: 951020729 |
25 |
VWR |
Reagents
Qiagen Nuclease-Free Water |
| Description/Quantity |
Ordering Information |
Supplier |
| 10 bottles of 50mL each |
Qiagen#: 129114 |
Qiagen |
| 1000mL |
Qiagen#: 129115 |
Qiagen |
| 5L |
Qiagen#: 129117 |
Qiagen |
Sources of Mouse BACs
(Bacterial Artificial Chromosomes) |
From Alex Bird, Max Planck Institute, Dresden.
"We order most of our BACs through 'BACPAC' http://bacpac.chori.org/ Sometimes we want a clone from a library they don't carry and then usually go through Invitrogen. To find BACs that contain a gene of interest, we often use the UCSC genome browser http://genome.ucsc.edu and turn on 'BAC end pairs'." |
Tips
Velocity11 Tips |
| Description |
Ordering Information |
Supplier |
| 96-tip racks, 200uL, non-sterile |
Part #: 06880.002
(reference Quote#: 28438) |
Velocity11 |
| 96-tip racks, 180uL, Sterile, Filtered |
Part#: 08585.002 |
Velocity11 |
| 384-tip racks, 30uL, non-sterile |
Part#: 11484.002 |
Velocity11 |
|
