Application for RNAi Screening (download this page as PDF)

ICCB-Longwood welcomes applications from academic investigators and NERCE affiliates. Applications must be submitted each time a new screen is proposed. We are currently accepting applications on a rolling basis. If the facility becomes oversubscribed we will institute a waiting list.

A fee is charged for access to RNAi screening at ICCB-Longwood. This fee helps us to recover some of our operating costs. It is revised annually on a schedule based on the Harvard fiscal year (July 1 - June 30), thus updates to the fee structure are generally posted here in the late summer and early fall. Please contact Caroline Shamu with questions about the schedule of implementation of new fee revisions. Note that as of October 2009 we have broken down the RNAi screening fees into increments that will be charged as RNAi screening projects progress.

ICCB-Longwood RNAi Screening Fees - updated October 1, 2009

Fees will begin to be assessed once work is underway in the ICCBL screening facility and the RNAi screening fee will not be assessed until screening of ICCBL RNAi libraries has started. For screens originating from Non-HMS Quad investigators (this includes HMS Affiliates whose labs are not located on the HMS Quadrangle and Non-Harvard screeners), the F&A fee will be assessed at the time the set-up fee is charged ($4,769 total set-up fee and F&A).

Interested screeners should contact Caroline Shamu for more details and/or to determine which rate applies to their screen. The fee is in addition to the cost of assay reagents and supplies provided by the screener. Please contact Caroline Shamu if you have any questions.

The above fees include cherry picks for 2000 individual siRNA duplexes (representing 500 genes) for each screen. Additional cherry picks can be requested at $1 per duplex picked ($4 per gene). Cherry picking will only be carried out for individual duplexes, not for SMARTpool reagents. Cherry picking can only be requested after primary screening of SMARTpool libraries.

For more information about working at ICCB-Longwood to carry out screening projects, please see our website, http://iccb.med.harvard.edu.

Often, investigators request a Letter of Collaboration for grants intended to fund a screen. Please understand that we can only write such a letter if we know that we can accommodate your screen. Therefore, please try to submit your Application to screen at the ICCB-Longwood well in advance of grant deadlines, and please warn us if you expect to need a Letter of Collaboration.

Please send any questions about the ICCB-Longwood Screening Application or evaluation process to screeniccb_apply"AT"hms.harvard.edu.

If you think you are having problems submitting an Application via this website, please send an explanatory email directly to screeniccb_apply@hms.harvard.edu or to the Director of ICCB-Longwood. Thank you.