Application for RNAi Screening at ICCB-Longwood
Application for RNAi Screening

Applications are currently being accepted on a rolling basis. Please note that, a fee will be charged for access to RNAi screening at ICCB-Longwood. Please see the application for screening page for more details about the fee structure.


Contact and Biographical Information:
Name:
Email:
Telephone #: - - (optional)
Fax #: - - (optional)
Lab Name:
(name of P.I.)
Lab Affiliation:
(Dept./Institution)
Applicant's position in the lab:
(e.g.lab head, postdoc, graduate student)

If you are not the lab head, do you plan to stay in your current lab for at least the next year? If not, briefly describe whether and how you plan to continue work on this screen in your next research position:

Research Plan:
1. Brief description of the pathway or biological process targeted by your proposed screen.
2. Please list one review article or other publication on the pathway/protein targeted by your screen (not necessarily authored by you) that would provide good background reading for the Review Committee.
3. Please provide some details about the assay you plan to use for screening and note whether or not it has already been carried out in 96- or 384-well format. If your assay is still being developed, please describe its status.
4. Do you have a control siRNA for your assay that mimics the phenotype of a desired screening positive? If yes, please describe.
5. Is it feasible for you to carry out your screen in duplicate?
6. Do you have secondary assays ready for further characterization of siRNAs that score as positives in your primary screen? Please describe at least two of them briefly and explain how they will help prioritize your hits.
7. Please give a realistic estimate as to when you think your assay will be ready to run pilot screens in the ICCB-Longwood screening facility. Please note whether or not you are waiting for funding to start this project.
8. If you require a Letter of Collaboration from ICCB-Longwood for a grant, please provide the grant deadline and the name of the granting agency to which you are applying.

A copy of your completed application will be sent automatically to the email address that you provided above.