CellProfiler Workshop, Friday December 11, 2009, 10am-3pm

you must register to attend, click here to register at the HMS Nikon Imaging Center Website.

Instructor : Mark-Anthony Bray, Ph.D, Computational Biologist/Image Assay Developer, Imaging Platform, The Broad Institute

Image-based screens increasingly require the use of automated microscopes which generates hundreds, if not thousands of images at a time. With such large image sets, automatic image analysis is more objective and quantitative and less tedious then visual inspection alone. This tutorial is meant to instruct biologists in the use of CellProfiler (http://www.cellprofiler.org/), an open-source, freely-downloadable software package designed for analyzing such images via a user-friendly interface. This course will begin with an introduction of CellProfiler, followed by details for the construction and use of a pipeline for analyzing typical image data. We will also include some of the more important considerations in cellular image analysis, by demonstrating the software on sample images as a realistic use case. Lastly, we will cover measurement export and preparation for additional analysis using machine-learning tools.