RNAi Transfection

ICCB-Longwood uses an integrated linear platform that includes the Agilent 4-R BenchCel, Bravo and PlateLoc. This setup, contained in a Baker BioProtect II biosafety cabinet, and the associated Thermo Multidrop Combi provides a precise and flexible platform allowing for a wide array of applications.

  • Just-in-time library delivery during the transfection step of RNAi screens
  • Approximately three minutes to transfer contents from one RNAi library plate to three assay plates
  • Reformat RNAi libraries
  • Custom ICCB-L projects

Agilent Bravo Automated Pipettor

  • 96- and 384-well heads enable transfer of RNAi reagents into 96- and 384-well microplates
  • Disposable tips are used to eliminate the potential for cross contamination (available from Agilent or Fluotics Ltd.)
  • A program is written and calibrated for each transfer, as the volume and number of replicates varies by screen 
  • Typical transfer volumes range between 1 – 3 uL per replicate
  • An ‘intermediate plate’ is used when transferring to more than 3 assay plates

Agilent Bravo automated pipettor

Agilent 4-R BenchCel Plate Handler

Delivers large numbers of library plates and tip boxes to and from the Bravo deck for processing. 

Agilent PlateLoc Heat Sealer

Quickly and effectively seals RNAi library plates after the transfer.