Fees

A fee is charged for access to RNAi screening at ICCB-Longwood. This fee helps us to recover a portion of our operating expenses. ICCB-Longwood screening fees (and associated Facilities and Administration fees) are revised annually based on the Harvard Fiscal Year (July 1 – June 30). New fees are typically recalculated in September or October of each year. The fee structure is updated here on the ICCB-Longwood website as soon as it is available. All charges are based on the screening fee at the time of service, not a previously provided estimate or price structure from a previous fiscal year.

Fees below apply to screening activities carried out in FY17 (July 1, 2016 – June 30, 2017). We anticipate these fees will be in effect until Fall 2017, at which time the FY18 fees should be calculated and available.

Note that fees charged for access to ICCB-Longwood do not include the cost of screening reagents and supplies. Funding for all screening reagents and supplies is provided by the screener.

Fees for HMS Internal Quad Screens

Screening Milestone
Fees
RNAi screen set-up fee
$3,226
Additional fee for screening one full genome siRNA library
$10,801
Additional fee for screening lncRNA siRNA library

$1,112

Additional fee for screening each miRNA reagent library

$794

In lieu of screening the full human genome library, it is possible to screen smaller, focused libraries: 
 Additional fee per library for screening: Phosphatases, Ubiquitin Conjugating Enzymes #1 or Ubiquitin Conjugating Enzymes #2$159
 Additional fee per library for screening: G-protein Coupled Receptors, Ion Channels, Proteases or Ubiquitin Conjugating Enzymes #3 $317.67
 Additional fee for screening: Kinases$476.51

The above full-genome screening fee includes cherry picks for 1,200 individual siRNA duplexes (representing 300 genes) for each screen. Additional cherry picks can be requested at $1.50 per duplex picked ($6 per gene). Cherry picks can only be requested for individual duplexes, not for SMARTpool reagents. It is not possible to request a cherry pick from Human Pilot 1.

Fees for HMS-Affiliated and Non-Harvard Academic Screens

Screening Milestone
Fees 
Corresponding F&A
RNAi screen set-up fee
$4,033
$972
Additional fee for screening one full genome siRNA library
$13,501
$3,255
Additional fee for screening lncRNA siRNA library

$1,390

$294

Additional fee for screening each miRNA reagent library

$993

$210

In lieu of screening the full human genome library, it is possible to screen smaller, focused libraries:
 Additional fee per library for screening: Phosphatases, Ubiquitin Conjugating Enzymes #1 or Ubiquitin Conjugating Enzymes #2$199 $42
 Additional fee per library for screening: G-protein Coupled Receptors, Ion Channels, Proteases or Ubiquitin Conjugating Enzymes #3$397 $84
 Additional fee for screening: Kinases $596 $126

The above full-genome screening fee includes cherry picks for 1,200 individual siRNA duplexes (representing 300 genes) for each screen. Additional cherry picks can be requested at $1.50 per duplex picked ($6 per gene). Cherry picks can only be requested for individual duplexes, not for SMARTpool reagents. It is not possible to request a cherry pick from Human Pilot 1.

Additional Information on RNAi Fees

Fees will begin to be assessed once work is initiated at ICCB-Longwood. For screens originating from non-HMS Quad investigator labs (this includes HMS Affiliates whose labs are not located on the HMS Quadrangle and Non-Harvard investigators), the indicated F&A is added at each stage of the screening fee. The fee for screening RNAi libraries will be assessed when the first library plate is screened.

The above fees for screening the full genome include cherry picks for the individual siRNA duplexes corresponding to 300 genes per screen (1200 duplexes for Dharmacon genome, 900 duplexes for an Ambion screen). Additional cherry picks can be requested at $1.50 per duplex picked ($6 per gene). Cherry picking will only be carried out for individual duplexes, not for SMARTpool reagents. Cherry picking can only be requested after primary screening of SMARTpool libraries. The duplex volume provided is the same volume of SMARTpool reagents that were used in the primary screen.

It is now possible for investigators to select whether they would like to screen the entire genome or instead screen focused libraries. For the human genome, this decision should be made prior to initiating the screen, as the most concise way to screen the entire genome is through utilizing the continuous human genome library. Custom library screening is also supported as possible.

Interested investigators should contact Jennifer Smith for more details and/or to determine which rate applies to their screen.