ICCB-Longwood has two quantitative PCR instruments
There is a training and annual access fee for the LifeTechnologies QuantStudio 7 qPCR instruments; please see the bottom of this page for additional information.
Quantitative PCR
LifeTechnologies QuantStudio 7
These instruments were purchased with funding from Harvard Medical School’s Tools and Technologies Committee (Flex) and Foundry (Pro).
The standard dyes these instruments are calibrated for are: FAM, VIC, ROX, SYBR, TAMRA, NED. If you are using a custom dye in your assay, please plan to calibrate your own protocol.
All experiments on these instruments are performed in 384-well microplates.
A document containing a brief QS7 Pro Software Tutorial for the new version of the software on the QS7 Pro is available for current QS7 Flex users.
For more information visit the ThermoFisher website.
qPCR Supplies
| Item | Cat. # | Supplier | Description |
|---|---|---|---|
| MicroAmp Optical 384-well Reaction Plate with Barcode | 4309849 | Life Technologies | PCR Compatible |
| MicroAmp Optical Adhesive Film | 4311971 | Life Technologies | 100 each |
| MicroAmp Optical Adhesive Film | 4360954 | Life Technolgies | 50 each |
QS7 Flex Filter Sets
| Filter set | Color | Excitation (nm) | Emission (nm) | Supported dye |
| x1-m1 | Blue | 470 ± 15 | 520±15 | FAM™ and SYBR® Green dyes |
| x2-m2 | Green | 520 ± 10 | 558 ± 12 | JOE™, HEX™, TET™, and VIC® dyes |
| x3-m3 | Yellow | 549.5 ± 10 | 586.5 ± 10 | Cy®3, NED™, and TAMRA™ dyes |
| x4-m4 | Orange | 580 ± 10 | 623 ± 14 | ROX™ and Texas Red® dyes |
| x5-m5 | Red | 640 ± 10 | 682 ± 14 | Cy®5 and LIZ® dyes |
| x6-m6 | Deep red | 662 ± 10 | 711 ± 12 | None |
QS7 Pro Filter Sets
| Filter set | Color | Excitation (nm) | Emission (nm) | Pre-calibrated dye | Example custom dyes |
| x1-m1 | Blue | 470 ± 15 | 520±15 | FAM™ and SYBR® Green | SYT09 |
| x2-m2 | Green | 520 ± 10 | 558 ± 12 | VIC® | HEX™, TET™, and JOE™ |
| x3-m3 | Yellow | 550 ± 10 | 587 ± 10 | ABY®, NED™, and TAMRA™ | Cy®3 |
| x4-m4 | Orange | 580 ± 10 | 623 ± 14 | JUN® and ROX™ | Texas Red® |
| x5-m5 | Red | 640 ± 10 | 682 ± 14 | Cy®5 and MUSTANG PURPLE® | LIZ® |
| x6-m6 | Deep-Red | 662 ± 10 | 711 ± 12 | None | Cy®5.5 |