Abstract
Human cytomegalovirus (HCMV) kinase UL97 is required for efficient nuclear lamina disruption during nuclear egress. However, cellular protein kinase C (PKC) has been implicated in this process in other systems. Comparing the effects of UL97 and cellular kinase inhibitors on HCMV nuclear egress confirms a role for UL97 in lamina disruption and nuclear egress. A pan-PKC inhibitor did not affect lamina disruption but did reduce the number of cytoplasmic capsids more than the number of nuclear capsids.
Copyright © 2014, American Society for Microbiology. All Rights Reserved.
Publication types
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Comparative Study
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Research Support, N.I.H., Extramural
MeSH terms
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Capsid / metabolism
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Cell Line
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Cell Nucleus / drug effects
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Cell Nucleus / virology*
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Cytomegalovirus / drug effects
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Cytomegalovirus / enzymology*
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Cytomegalovirus / genetics
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Cytomegalovirus / physiology
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Cytomegalovirus Infections / enzymology*
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Cytomegalovirus Infections / virology
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Humans
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Nuclear Lamina / drug effects
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Nuclear Lamina / virology*
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Phosphotransferases (Alcohol Group Acceptor) / antagonists & inhibitors*
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Phosphotransferases (Alcohol Group Acceptor) / genetics
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Phosphotransferases (Alcohol Group Acceptor) / metabolism
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Protein Kinase C / antagonists & inhibitors*
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Protein Kinase C / metabolism
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Protein Kinase Inhibitors / pharmacology*
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Virus Assembly / drug effects
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Virus Release / drug effects*
Substances
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Protein Kinase Inhibitors
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Phosphotransferases (Alcohol Group Acceptor)
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ganciclovir kinase
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Protein Kinase C