Comparison of effects of inhibitors of viral and cellular protein kinases on human cytomegalovirus disruption of nuclear lamina and nuclear egress

J Virol. 2014 Sep;88(18):10982-5. doi: 10.1128/JVI.01391-14. Epub 2014 Jun 25.

Abstract

Human cytomegalovirus (HCMV) kinase UL97 is required for efficient nuclear lamina disruption during nuclear egress. However, cellular protein kinase C (PKC) has been implicated in this process in other systems. Comparing the effects of UL97 and cellular kinase inhibitors on HCMV nuclear egress confirms a role for UL97 in lamina disruption and nuclear egress. A pan-PKC inhibitor did not affect lamina disruption but did reduce the number of cytoplasmic capsids more than the number of nuclear capsids.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Capsid / metabolism
  • Cell Line
  • Cell Nucleus / drug effects
  • Cell Nucleus / virology*
  • Cytomegalovirus / drug effects
  • Cytomegalovirus / enzymology*
  • Cytomegalovirus / genetics
  • Cytomegalovirus / physiology
  • Cytomegalovirus Infections / enzymology*
  • Cytomegalovirus Infections / virology
  • Humans
  • Nuclear Lamina / drug effects
  • Nuclear Lamina / virology*
  • Phosphotransferases (Alcohol Group Acceptor) / antagonists & inhibitors*
  • Phosphotransferases (Alcohol Group Acceptor) / genetics
  • Phosphotransferases (Alcohol Group Acceptor) / metabolism
  • Protein Kinase C / antagonists & inhibitors*
  • Protein Kinase C / metabolism
  • Protein Kinase Inhibitors / pharmacology*
  • Virus Assembly / drug effects
  • Virus Release / drug effects*

Substances

  • Protein Kinase Inhibitors
  • Phosphotransferases (Alcohol Group Acceptor)
  • ganciclovir kinase
  • Protein Kinase C