Pin Transfer

Pin Array

ICCB-Longwood utilizes two different robotic platforms to conduct pin transfer of nanoliter volumes from compound storage plates into assay plates using stainless steel pin arrays (made by V&P Scientific).

  • 96- and 384-well pin arrays
  • Transfer compounds into 96-, 384- and 1536-well microplates
  • 384-well assay plate volume 30 uL - calibrated to transfer 33 nL, 100 nL or 300 nL
  • 384-well assay plate volume 10 uL - calibrated to transfer 33 nL or 100 nL
  • The standard transfer volume for chemical screens in 384-well is 100 nL.  
  • 96-well assay plate volume 150 uL - calibrated to transfer 200 nL, 250 nL or 300 nL

Pin Arrays & Calibration provides more information on the pin arrays, the calibration process and selecting the appropriate pin array for your screen.

Seiko Compound Transfer Robot (SGM 611)

Seiko Compound Transfer Robot

  • Cartesian (linear) robot custom designed and built at ICCB-Longwood
  • Paired with an Epson 6-axis robot arm to deliver library and assay plates to and from the deck for transfer
  • Integrated with a Symbol Miniscan barcode reader for compound transfer tracking
  • Takes approximately one minute to transfer compound to one assay plate
  • Can automatically attach and detach the pin tools
Design & Construction Original design: Randall King and Leslie Walling
Current design: Stewart Rudnicki, Sean Johnston, & Doug Flood
Visual Basic Interface  Custom software designed by ICCB-Longwood personnel
Robotic Arms
  • Seiko D-TRAN XM3106-31 PN 4-axis cartesian robot controlled by SRC-310A controller
  • Epson C3-A601S 6-axis robot controlled by an RC-620 controller
  • Programmed with SPEL for Windows
Table Custom Designed by Newport Scientific
Sonicator Sonicor SC-50
Methanol Bath Designed by ICCB-Longwood personnel
Dry Station Designed by ICCB-Longwood personnel
Pin Arrays

Manufactured by V&P Scientific.  

  • 33nL
  • 100nL
  • 300nL
Cylce Time Approximately 1 minute per transfer, including wash time
Plate Types Accepted 96-well, 384-well

The amount of compound transferred is dependent on several parameters

  • Pin shape and diameter
  • Volume of stock solution
  • Rate of pin withdrawal from the stock solution
  • Viscosity of liquid in the assay plate
  • Dwell time in the assay plate

To prevent compound carryover, each pin array is washed after every library transfer.  

Pin Washing Steps:

  • PBS
  • 100% methanol flow-through wash
  • 20% methanol sonication step
  • (repeat twice)
  • Concludes with a 10-second high-pressure air dry

Epson Compound Transfer Robot (SGM 612)

Epson Robot

  • Epson E2C2515-UL Scara robot custom designed and built at ICCB-Longwood
  • Paired with an Epson 6-axis robot arm to deliver library and assay plates to and from the deck for transfer
  • Integrated with a Symbol Miniscan barcode reader for compound transfer tracking. 
  • Takes approximately one minute to transfer compound to one assay plate
  • Can automatically attach and detach the pin tool
Design & Construction Stewart Rudnicki, Sean Johnston, & Doug Flood
Visual Basic Interface  Custom software designed by ICCB-Longwood personnel
Robotic Arms
  • Epson E2C2515-UL Scara robot, controlled by a RC-410 controller.
  • Epson C3-A601S 6-axis robot controlled by an RC-620 controller
  • Programmed with SPEL for Windows
Table Custom Designed by Jim Horn
Sonicator Cole Parmer Ultrasonic Cleaner
Methanol Bath Designed by ICCB-Longwood personnel
Dry Station Designed by ICCB-Longwood personnel
Pin Arrays

Manufactured by V&P Scientific.  

  • 33nL
  • 100nL
  • 300nL
Cylce Time Approximately 1 minute per transfer, including wash time
Plate Types Accepted 384-well

The amount of compound transferred is dependent on several parameters

  • Pin shape and diameter
  • Volume of stock solution
  • Rate of pin withdrawal from the stock solution
  • Viscosity of liquid in the assay plate
  • Dwell time in the assay plate

To prevent compound carryover, each pin array is washed after every library transfer.  

Pin Washing Steps:

  • PBS
  • 100% methanol flow-through wash
  • 20% methanol sonication step
  • (repeat twice)
  • Concludes with a 10-second high-pressure air dry