RNAi Libraries

RNAi Libraries

The stock concentration for the siRNA library plates designated for screening is 1 µM.  All of the siRNAs are stored in 1x Dharmacon siRNA buffer (20 mM KCl, 6 mM HEPES-pH 7.5, and 0.2 mM MgCl2).  The below plate numbers are for 384-well plates.  Screeners using 96-well plates for their assay will be provided with the appropriate information to translate 384-well plate numbers to 96-well plate numbers for the purposes of labeling assay plates.

A diagram showing how four 96-well plates map to one 384-well plate is available as a PDF here.

Please note that we are not in a position to supply reagents to investigators not screening with ICCB-Longwood. However, we provide information about the vendors from whom these reagents can be acquired.

Jump to Library Type:

Dharmacon 2014 Human Pools – Continuous Human Genome
Dharmacon 2014 Human siRNA Pools – Focused Libraries
Dharmacon 2014 Human lncRNA siRNA Pools
Dharmacon 2006 Mouse siRNA Pools
Human miRNA Collections
Dharmacon 2006 Human siRNA Pools
Ambion Human siRNA Pools
Sigma Human esiRNA Pools
Invitrogen Human siRNA Pools


Dharmacon 2014 Human siRNA Pools – Continuous Human Genome

Library Name
Number of siRNA Wells
Number of Plates
ICCB-L Plate Numbers
Dharmacon 2014 Human 6: Druggable Genome Compiled 9-16 Focused Libraries 2,785 10 51,144-51,153
Dharmacon 2014 Human 7: Remaining Druggable Genome 4,853 18 51,154-51,171
Dharmacon 2014 Human 8: Remaining Genome 10,582 38 51,172-51,209
ICCB-L Pilot 1 237 1 51,083

Click on a library name to view additional information and 384-well plate layout.


Dharmacon 2014 Human siRNA Pools – Focused Libraries

Library Name
Number of siRNA Wells
Number of Plates
ICCB-L Plate Numbers
Dharmacon 2014 Human 9: Focused Library of G-protein Coupled Receptors 390 2 51,210-51,211
Dharmacon 2014 Human 10: Focused Library of Kinases 720 3 51,212-51,214
Dharmacon 2014 Human 11: Focused Library of Ion Channels 349 2  51,215-51,216
Dharmacon 2014 Human 12: Focused Library of Proteases  480 2 51,217-51,218
Dharmacon 2014 Human 13: Focused Library of Phosphatases  256 1 51,219
Dharmacon 2014 Human 14: Focused Library of Ubiquitin Conjugating Enzymes #1  89 1 51,220
Dharmacon 2014 Human 15: Focused Library of Ubiquitin Conjugating Enzymes #2  115 1 51,221
Dharmacon 2014 Human 16: Focused Library of Ubiquitin Conjugating Enzymes #3  390 2 51,222-51,223

Click on a library name to view additional information and 384-well plate layout.


Dharmacon 2014 Human lncRNA siRNA Pools

Library Name
Number of siRNA Wells
Number of Plates
ICCB-L Plate Numbers
Dharmacon 2014 Human 17: Lincode NR Long-noncoding RNA 2,231 7 51,224-51,230


Dharmacon Mouse siRNA Pools

Library Name
Number of siRNA Wells
Number of Plates
ICCB-L Plate Numbers
Dharmacon Mouse 1: Kinases-Phosphatases 719 3 50,025-50,027
Dharmacon Mouse 2: GPCRs 474 2 50,028-50,029
Dharmacon Mouse 3: Remaining Druggable Genome 5,170 17 50,030-50,046
Dharmacon Mouse 4: Remaining Genome 10,510 33 50,440-50,472
Dharmacon Mouse 5: Supplemental Library 3,679 14 51,084-51,097

Click on a library name to view additional information and 384-well plate layout.


Human miRNA Collections

Library Name
Number of miRNA Wells
Number of Plates
ICCB-L Plate Numbers
Qiagen Human 1: Mimics 875  5 50,684-50,688
Ambion Human 1: Mimics 885  5 50,689-50,693
Exiqon Human 1: Inhibitors 918  5 50,694-50,698

Click on a library name to view additional information and 384-well plate layout.


Dharmacon 2006 Human siRNA Pools

Library Name
Number of siRNA Wells
Number of Plates
ICCB-L Plate Numbers
Dharmacon Human 1: Kinases-Phosphatases 779 3 50,001-50,003
Dharmacon Human 2: GPCRs 516 2 50,004-50,005
Dharmacon Human 3: Remaining Druggable Genome 6,022 19 50,006-50,024
Dharmacon Human 4: Remaining Genome 13,804 44 50,047-50,090
Dharmacon Human 5: RefSeq27 Reversion 4,506 17 51,008-51,024
*Mitchison Kinase 1 509 2 50,091-50,092
ICCB-L Pilot 1 237 1 51,083
Dharmacon Human DUB 1 127 1 50,439

Click on a library name to view additional information and 384-well plate layout.

*Retired library


Ambion Human siRNA Pools

Library Name
Number of siRNA Wells
Number of Plates
ICCB-L Plate Numbers
Ambion Human 1: Druggable Genome 10,414 36 50,711-50,746
Ambion Human 2: Remaining Genome 11,170 38 50,747-50,784


Sigma Human esiRNA Pools

Library Name
Number of siRNA Wells
Number of Plates
ICCB-L Plate Numbers
Sigma esiRNA Human 1 12,000 42 50,642-50,683
Sigma esiRNA Human 2 3,300 12 50,699-50,710


Invitrogen Human siRNA Pools

Library Name
Number of siRNA Wells
Number of Plates
ICCB-L Plate Numbers
Invitrogen Human 1: Stealth Kinases-Phosphatases 2,619 11 50,611-50,621
Invitrogen Human 2: Stealth Remaining Druggable 4,455 19 50,622-50,641
Invitrogen Human 3: QC Supplement 249 1 51,082