ImageXpress Micro

Molecular Devices ImageXpress Micro

  • An inverted fully automated epifluorescent microscope designed for scanning standard multi-well microplates or slides.
  • Compatible with 6-, 12-, 24-, 48-, 96-, 384- and 1536-well plates.
  • Laser auto-focus option that allows for the acquisition of up to 50,000 wells (200 384-well plates) per day depending on the number of sites and wavelengths imaged per well.
  • Equipped with the Photometrics CoolSNAP ES digital CCD camera.

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Designing an Image-Based Screen

Assay plates:

Black-walled, clear-bottom (plastic or glass) microplates are used for image-based screens. A common 384-well plate type is Corning #3764 (previously #3712).

Seeding density:

Test a variety of cell densities under the conditions you plan to use for the screen. Image analysis is often improved when the cells are sub-confluents. Depending upon cell size and length of assay, a starting range may be 500 to 2,000 cells per well.

Imaging live cells:

This is possible when using the Acumen. Remember that the microscope stage is not heated and your cells will be at room temperature during imaging.

Treating plates after imaging:

After processing cells for imaging, fill your wells with PBS to prevent the cells from drying out and to reduce background noise. Some investigators include an anti-fungal agent (eg sodium azide) for long term storage at 4oC.

Selecting fluorophores:

Please make sure the fluorophores you select do not conflict with the filter sets on the screening scope. See details for each microscope. For high throughput screens, antibodies are frequently used at higher concentrations to decrease acquisition time.

ImageXpress Objective Options

Magnification Objective Type NA (uM) WD (mm)
4X S Fluor 0.20 15.5
10X Plan Fluor 0.30 16
20X Plan Fluor ElWD 0.45 8.1-7.0
40X Plan Fluor ElWD 0.60 3.7-2.7

ImageXpress Filters

Filters Excitation Emission Dichroic
DAPI 377/50  447/60 344-404 reflection
CFP 438/24 483/32 458
GFP 472/30 520/35 495
FITC 482/35 536/40 446-500 reflection
YFP 500/24 542/27 520
Cy3 531/40 593/40 495-555 reflection
Texas Red 562/40 624/40 530-585 reflection
Cy5 628/40 692/40 594-651 reflection