EnVision
Perkin Elmer EnVision plate reader
ICCB-Longwood has three EnVision plate readers that can be run in single plate mode or with a detachable 40-plate stacker.
A document outlining Assay Optimization for the EnVision is available for interested users.
Multimode Plate Reader Capabilities
- Absorbance (230-1100 nm)
- Fluorescence (230-1100 nm)
- Luminescence
- Enhanced Luminescence
- Time-Resolved Fluorescence
- Fluorescence Polarization
- DELFIA (TRF for heterogeneous assays)
- LANCE (TR-FRET for homogeneous assays)
- NanoBRET
EnVision 1
Located in SGM 611
This EnVision was purchased in 2021 with support from HMS Foundry
- It is equipped with monochromators, enabling tunability of excitation and emission wavelengths for top absorbance and fluorescence intensity quantitation.
- It has a dedicated laser (337 nm) for HTRF.
- This EnVision has HTS 'Turbo' for AlphaScreen assays.
EnVision 2
Located in SGM 612
Provides HTS "Turbo" AlphaScreen. The purchase of this new model was supported in part by the Harvard Medical School Tools and Technologies Committee.
EnVision 1 Filters & Mirrors
|
Filter Set |
Wavelength (nm) |
Bandwidth (nm) |
Label |
Type |
Description |
Bar Code |
| Monochromator* | 585 | X585co | Filter | Monochromator cutoff 585 | 142 | |
| 360 | X360co | Filter | Monochromator cutoff 360 | 141 | ||
| N/A | 405 | 8 | Filter | Photometric 405 | 302 | |
| N/A | n/a | BS50/BS50 | Mirror | Dual beamsplitter enhanced | 651 | |
| NanoBRET | 585 | Mirror | LUM/D585 | 673 | ||
| 460 | 80 | 460/80 | Filter | NanoBRET Blue | 703 | |
| 647 | 75 | 647/75 | Filter | BRET Deep Red 647 | 704 | |
| FITC FP Dual | 505/535 | Mirror | D505FP/D535 | 681 | ||
| 480 | 30 | X480 | Filter | FITC FP 480 | 104 | |
| 535 | 40 | M535p | Filter | FITC FP P-pol 535 | 209 | |
| 535 | 40 | M535s | Filter | FITC FP S-pol 535 | 208 | |
| BODIPY TMR FP | 555/595 | Mirror | D555fp/D595 | 682 | ||
| 595 | 60 | M595p | Filter | Bodipy TMR P-pol | 211 | |
| 595 | 60 | M595s | Filter | Bodipy TMR S-pol | 210 | |
| 531 | 25 | X531 | Filter | Bodipy TMR FP | 106 | |
| LANCE LASER | 407/630 | Mirror | TRF Laser Lance | 667 | ||
| 407/505 | Mirror | TRF Laser Lance | 665 | |||
| 615 | 8 | M615a | Filter | LANCE Laser attenuated Europium filter | 249 | |
| 665 | 7.5 | M665 | Filter | APC 665 | 205 |
* required for absorbance and fluorescence intensity top reads
EnVision 2 & 3 Filters
Filters
|
Wavelength/ |
Label
|
Description
|
EnVision |
EnVision |
Bar Code
|
|
270/15
|
Custom 270/15
|
x
|
|
513
|
|
|
330/80
|
Custom 330
|
x
|
xx
|
501
|
|
|
340/60
|
P340
|
UV (TRF) 340
|
x
|
101
|
|
|
340/25
|
P340
|
Photometric 340
|
x
|
x |
301
|
|
340/30
|
Custom 340
|
x
|
xx
|
502
|
|
|
355/40
|
X355
|
Umbelliferone 355
|
x
|
xx
|
103 |
|
380/30
|
Custom 380
|
x
|
x
|
503
|
|
|
405/8
|
P405
|
Photometric 405
|
xxx
|
xx
|
302
|
|
420/8
|
P420
|
Photometric 420
|
x
|
xx
|
308
|
|
460/25
|
M460
|
Umbelliferone 460
|
x
|
xx
|
207
|
|
470/40
|
D595
|
Custom GFP 470/40
|
x
|
xx
|
504
|
|
475/8
|
P475
|
Photometric 475
|
x
|
xx
|
309
|
|
480/30
|
X480
|
FITC FP 480
|
x
|
xx
|
104
|
|
485/14
|
X485
|
FITC 485
|
x
|
xx
|
102
|
|
495/10
|
Custom 495
|
x
|
xx
|
505
|
|
|
510/10
|
X510
|
YFP 510
|
xx
|
x
|
114
|
|
515/30
|
M515
|
BRET2 515
|
x
|
x
|
219
|
|
520/25
|
Custom 520
|
x
|
xx
|
506
|
|
|
525/50
|
Custom 525
|
x
|
xx
|
507
|
|
|
530/10
|
Mm530
|
YFP 530
|
|
x |
221
|
|
531/25
|
X531
|
BODIPY TMR FP 531
|
xxx
|
xxxx
|
105
|
|
535/25
|
M535
|
FITC 535
|
xx
|
x
|
206
|
|
535/40
|
M535s
|
FITC FP S-POL (Em)
|
x
|
xx
|
208
|
|
535/40
|
M535p
|
FITC FP P-POL (Em)
|
x
|
x
|
209
|
|
550/9
|
P550
|
Photometric 550
|
x |
312
|
|
|
570/100
|
M570w
|
Alphascreen 570
|
x |
244
|
|
|
570/8
|
P570
|
Photometric 570
|
xx
|
x
|
318
|
|
580/10
|
M580
|
Emission 580
|
x
|
xx
|
233
|
|
580/40
|
Custom 580
|
x
|
x
|
508
|
|
|
590/20
|
M590
|
Rhodamine 590
|
x
|
xx
|
217
|
|
595/60
|
M595s
|
BODIPY TMR FP S-POL 595
|
x
|
x |
210
|
|
595/60
|
M595p
|
BODIPY TMR FP P-POL 595
|
xx
|
x |
211
|
|
595/60
|
M595w
|
Cy3 595
|
x
|
xxx
|
229
|
|
600/8
|
P600
|
Photometric 600
|
x
|
xx
|
319
|
|
615/8.5
|
|
Europium 615
|
x
|
|
203
|
|
620/10
|
X620
|
Cy5 620
|
x
|
x
|
118
|
|
640/40
|
Custom 640
|
x
|
x
|
509
|
|
|
650/8
|
Emission 650
|
x
|
xx
|
227
|
|
|
650/50
|
Custom 650
|
x
|
x
|
510
|
|
|
665/7.5
|
M665
|
APC 665
|
x
|
x |
205
|
|
670/50
|
|
Custom 670/50
|
|
x
|
511
|
|
685/35
|
|
Cy5 685
|
xx
|
xx
|
243
|
|
715/20
|
|
Custom 715/20
|
x
|
|
512
|
|
720/8
|
P720
|
Photometric 720
|
x
|
x
|
322
|
|
n/a
|
LUM
|
Luminescence 700
|
x
|
x |
212
|
Filters with the narrowest bandwidth are recommended for photometry. If additional filters with narrow bandwidths are required, please see screening facility staff.
EnVision 2 & 3 Mirrors
Mirrors
|
Wavelength (nm)
|
Label
|
Description
|
EnVision 2 | EnVision 3 |
Bar Code
|
|
400
|
D400
|
LANCE/DELFIA
|
x |
412
|
|
|
495
|
D495 |
Custom 495
|
xx
|
404
|
|
|
505
|
D505
|
FITC
|
x
|
x
|
403 |
|
505
|
D505FP
|
FITC FP Single
|
x
|
431
|
|
|
505/535
|
D505fp/D535
|
FITC FP dual
|
x
|
x
|
481
|
|
555/595
|
D555fp/D595
|
BODIPY TMR FP dual
|
x
|
x |
682
|
|
555
|
D555
|
BODIPY TMR
|
xx
|
x
|
405
|
|
595
|
D595
|
Custom 595 |
x
|
404
|
|
|
640
|
D640as
|
Alphascreen
|
x |
444
|
|
|
658
|
D658
|
Cy5
|
x
|
xx |
420
|
|
700
|
Custom 700dcxr
|
Custom 700dcxr
|
x
|
404
|
|
|
n/a
|
BS50
|
Single beamsplitter
|
x
|
401
|
|
|
n/a
|
BS50/BS50
|
Dual beamsplitter
|
x
|
x |
451
|
|
n/a
|
BS50/BS50
|
Dual beamsplitter enhanced
|
x |
x
|
651
|