EnVision

Perkin Elmer EnVision plate reader

ICCB-Longwood has three EnVision plate readers that can be run in single plate mode or with a detachable 40-plate stacker.

A document outlining Assay Optimization for the EnVision is available for interested users. 

 

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Multimode Plate Reader Capabilities

  • Absorbance (230-1100 nm)
  • Fluorescence (230-1100 nm)
  • Luminescence
  • Enhanced Luminescence
  • Time-Resolved Fluorescence
  • Fluorescence Polarization
  • DELFIA® (TRF for heterogeneous assays)
  • LANCE® (TR-FRET for homogeneous assays)

Perkin Elmer EnVision plate reader

ICCB-Longwood has three EnVision plate readers that can be run in single plate mode or with a detachable 40-plate stacker.

A document outlining Assay Optimization for the EnVision is available for interested users. 

Request Training

Schedule Equipment

EnVision 2

Located in SGM 612

Provides HTS "Turbo" AlphaScreen. The purchase of this new model was supported in part by the Harvard Medical School Tools and Technologies Committee. 

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EnVision 3

Located in SGM 612

This instrument is equipped with only one detector and is therefore slower for reading dual read assays, such as fluorescence polarization.

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EnVision Filters

Filters

Wavelength/
Bandpass (nm)

Label
Description

EnVision
1

EnVision
2

EnVision
3

Bar Code
330/80
Custom 330
x
x
x
501
340/25
P340
Photometric 340
x
x
301
340/30
Custom 340
x
x
x
502
355/40
X355
Umbelliferone 355
x
x
x

103

380/30
Custom 380
x
x
x
503
405/8
P405
Photometric 405
x
xxx
x
302
420/8
P420
Photometric 420
x
x
x
308
460/25
M460
Umbelliferone 460
x
x
x
207
470/40
D595
Custom GFP 470/40
x
x
x
504
475/8
P475
Photometric 475
x
x
x
309
480/30
X480
FITC FP 480
x
x
x
104
485/14
X485
FITC 485
x
x
x
102
495/10
Custom 495
x
x
x
505
510/10
X510
YFP 510
x
x
x
114
515/30
M515
BRET2 515
x
x
x
219
520/25
Custom 520
x
x
x
506
525/50
Custom 525
x
x
x
507
530/10
Mm530
YFP 530
x
x
221
531/25
X531
BODIPY TMR FP 531
xx
xx
xx
105
535/25
M535
FITC 535
x
x
x
206
535/40
M535s
FITC FP S-POL (Em)
x
x
x
208
535/40
M535p
FITC FP P-POL (Em)
x
x
x
209
550/9
P550
Photometric 550
x
312
570/100
M570w
Alphascreen 570
x
244
570/8
P570
Photometric 570
x
x
x
318
580/10
M580
Emission 580
x
x
x
233
580/40
Custom 580
x
x
x
508
590/20
M590
Rhodamine 590
x
x
x
217
595/40
M595s
BODIPY TMR FP S-POL 595
x
x
210
595/40
M595p
BODIPY TMR FP P-POL 595
x
x
211
595/60
M595w
Cy3 595
x
x
x
229
600/8
P600
Photometric 600
x
x
x
319
620/10
X620
Cy5 620
x
x
xx
118
640/40
Custom 640
x
x
x
509
650/8
Emission 650
x
x
x
227
650/40
Custom 650
x
x
x
510
665/7.5
M665
APC 665
x
x
205
720/8
P720
Photometric 720
x
x
x
322
n/a
LUM
Luminescence 700
x
x
212

Filters with the narrowest bandwidth are recommended for photometry. If additional filters with narrow bandwidths are required, please see screening facility staff.

EnVision Mirrors

Mirrors

Wavelength (nm)
Label
Description
EnVision 1 EnVision 2 EnVision 3
Bar Code
400
D400
LANCE/DELFIA
x
 x  x
412
495
Special GFP
 x
x
404
505
D505
FITC
x
x
x

403

505
D505FP
FITC FP Single
x
431
505/535
D505fp/D535
FITC FP dual
x
x
x
481
555/595
D555fp/D595
BODIPY TMR FP dual
x
 x
682
555
D555
BODIPY TMR
x
x
x
405
595
D595
Standard Luminescence
 x
x
404
640
D640as
Alphascreen
x
444
658
D658
Cy5
x
x
 x
420
n/a
BS50
Single beamsplitter
x
401
n/a
BS50/BS50
Dual beamsplitter
x
x
451
n/a
BS50/BS50
Dual beamsplitter enhanced
 x
x
651